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The distribution pattern of ZO\u20101 differs among stratified squamous epithelia, including that between skin and oral buccal mucosa. However, the causes for this difference, and the mechanisms underlying ZO\u20101 spatial regulation, have yet to be elucidated. In this study, we showed that epithelial turnover and proliferation are associated with ZO\u20101 distribution in squamous epithelia. We tried to verify the regulation of ZO\u20101 by comparing normal skin and psoriasis, known as inflammatory skin disease with rapid turnover. We as well compared buccal mucosa and oral lichen planus, known as an inflammatory oral disease with a longer turnover interval. The imiquimod (IMQ) mouse model, often used as a psoriasis model, can promote cell proliferation. On the contrary, we peritoneally injected mice mitomycin C, which reduces cell proliferation. We examined whether IMQ and mitomycin C cause changes in the distribution and appearance of ZO\u20101. Human samples and mouse pharmacological models revealed that slower epithelial turnover\/proliferation led to the confinement of ZO\u20101 to the uppermost part of squamous epithelia. In contrast, ZO\u20101 was widely distributed under conditions of faster cell turnover\/proliferation. Cell culture experiments and mathematical modelling corroborated these ZO\u20101 distribution patterns. These findings demonstrate that ZO\u20101 distribution is affected by epithelial cell dynamics.<\/jats:p>","DOI":"10.1111\/cpr.13441","type":"journal-article","created":{"date-parts":[[2023,3,15]],"date-time":"2023-03-15T06:41:45Z","timestamp":1678862505000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Zonula occludens\u20101 distribution and barrier functions are affected by epithelial proliferation and turnover rates"],"prefix":"10.1111","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8855-3288","authenticated-orcid":false,"given":"Keisuke","family":"Imafuku","sequence":"first","affiliation":[{"name":"Department of Dermatology, Faculty of Medicine and Graduate School of Medicine Hokkaido University  Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroaki","family":"Iwata","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Faculty of 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