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Whether RNA N6\u2010methyladenosine (m\n                    <jats:sup>6<\/jats:sup>\n                    A) affects the developmental competency of SCNT embryos remains unclear. Here, we showed that porcine bone marrow mesenchymal stem cells (pBMSCs) presented higher RNA m\n                    <jats:sup>6<\/jats:sup>\n                    A levels than those of porcine embryonic fibroblasts (pEFs). SCNT embryos derived from pBMSCs had higher RNA m\n                    <jats:sup>6<\/jats:sup>\n                    A levels, cleavage, and blastocyst rates than those from pEFs. Compared with pEFs, the promoter region of METTL14 presented a hypomethylation status in pBMSCs. Mechanistically, DNA methylation regulated METTL14 expression by affecting the accessibility of transcription factor SP1 binding, highlighting the role of the DNA methylation\/SP1\/METTL14 pathway in donor cells. Inhibiting the DNA methylation level in donor cells increased the RNA m\n                    <jats:sup>6<\/jats:sup>\n                    A level and improved the development efficiency of SCNT embryos. Overexpression of METTL14 significantly increased the RNA m\n                    <jats:sup>6<\/jats:sup>\n                    A level in donor cells and the development efficiency of SCNT embryos, whereas knockdown of METTL14 suggested the opposite result. Moreover, we revealed that RNA m\n                    <jats:sup>6<\/jats:sup>\n                    A\u2010regulated\n                    <jats:italic>TOP2B<\/jats:italic>\n                    mRNA stability, translation level, and DNA damage during SCNT embryo development. Collectively, our results highlight the crosstalk between RNA m\n                    <jats:sup>6<\/jats:sup>\n                    A and DNA methylation, and the crucial role of RNA m\n                    <jats:sup>6<\/jats:sup>\n                    A during nuclear reprogramming in SCNT embryo development.\n                  <\/jats:p>","DOI":"10.1111\/cpr.13581","type":"journal-article","created":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T06:33:54Z","timestamp":1702535634000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["<scp>DNA<\/scp>\n                    methylation regulates\n                    <scp>\n                      RNA m\n                      <sup>6<\/sup>\n                      A\n                    <\/scp>\n                    modification through transcription factor\n                    <scp>SP1<\/scp>\n                    during the development of porcine somatic cell nuclear transfer embryos"],"prefix":"10.1111","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1800-0928","authenticated-orcid":false,"given":"Meng","family":"Zhang","sequence":"first","affiliation":[{"name":"Key Laboratory of Organ Regeneration and Transplantation of Ministry of Education The First Hospital of Jilin University  Changchun Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanhui","family":"Zhai","sequence":"additional","affiliation":[{"name":"Key Laboratory of Organ Regeneration and Transplantation of Ministry of Education The First Hospital of Jilin University  Changchun Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinglan","family":"An","sequence":"additional","affiliation":[{"name":"Key Laboratory of Organ Regeneration and Transplantation of Ministry of Education The First Hospital of Jilin University  Changchun Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Organ Regeneration and Transplantation of Ministry of Education The First Hospital of Jilin University  Changchun Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daoyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Organ Regeneration and Transplantation of Ministry of Education The First Hospital of Jilin University  Changchun Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongfeng","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Organ Regeneration and Transplantation of Ministry of Education The First Hospital of Jilin University  Changchun Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Organ Regeneration and Transplantation of Ministry of Education The First Hospital of Jilin University  Changchun Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4252-3195","authenticated-orcid":false,"given":"Xiangpeng","family":"Dai","sequence":"additional","affiliation":[{"name":"Key Laboratory of Organ Regeneration and Transplantation of Ministry of Education The First Hospital of Jilin University  Changchun Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8815-5696","authenticated-orcid":false,"given":"Ziyi","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Organ Regeneration and Transplantation of Ministry of Education The First Hospital of Jilin University  Changchun Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,12,14]]},"reference":[{"issue":"3","key":"e_1_2_10_2_1","first-page":"S13","article-title":"Animals, science, and ethics\u2014section III. 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