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Such antiresorptive properties of root relate to cementocyte mineralization, the mechanisms of which remain largely unknown. This study used the microarray analysis to explore long non\u2010coding ribonucleic acids involved in stress\u2010induced cementocyte mineralization. Gain\u2010 and loss\u2010of\u2010function experiments, including Alkaline phosphatase (ALP) activity and Alizarin Red S staining, quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR), Western blot, and immunofluorescence analyses of mineralization\u2010associated factors, were conducted to verify long non\u2010coding ribonucleic acids taurine\u2010upregulated gene 1 (LncTUG1) regulation in stress\u2010induced cementocyte mineralization, via targeting the Toll\u2010like receptor 4 (TLR4)\/SphK1 axis. The luciferase reporter assays, chromatin immunoprecipitation assays, RNA pull\u2010down, RNA immunoprecipitation, and co\u2010localization assays were performed to elucidate the interactions between LncTUG1, PU.1, and TLR4. Our findings indicated that LncTUG1 overexpression attenuated stress\u2010induced cementocyte mineralization, while blocking the TLR4\/SphK1 axis reversed the inhibitory effect of LncTUG1 on stress\u2010induced cementocyte mineralization. The in vivo findings also confirmed the involvement of TLR4\/SphK1 signalling in cementocyte mineralization during OTM. Mechanistically, LncTUG1 bound with PU.1 subsequently enhanced TLR4 promotor activity and thus transcriptionally elevated the expression of TLR4. In conclusion, our data revealed a critical role of LncTUG1 in regulating stress\u2010induced cementocyte mineralization via PU.1\/TLR4\/SphK1 signalling, which might provide further insights for developing novel therapeutic strategies that could protect roots from resorption during OTM.<\/jats:p>","DOI":"10.1111\/cpr.13604","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T05:50:11Z","timestamp":1707285011000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Long non\u2010coding <scp>RNA LncTUG1<\/scp> regulates favourable compression force\u2010induced cementocytes mineralization via <scp>PU<\/scp>.1\/<scp>TLR4<\/scp>\/<scp>SphK1<\/scp> signalling"],"prefix":"10.1111","volume":"57","author":[{"given":"Han","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral Diseases &amp; National Center for Stomatology &amp; National Clinical Research Center 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