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Drug treatment is a common clinical treatment that aims to increase bone mass and bone density. Tetrahedral DNA nanostructures (TDNs) are three\u2010dimensional tetrahedral frames formed by folding four single\u2010stranded DNA molecules, which have good biological safety and can promote bone regeneration. In this study, a mouse model of OP was established by ovariectomy (OVX) and TDN was injected into the tail vein for 8\u2009weeks. We found that ovariectomized mice could simulate some physiological changes in OP. After treatment with TDNs, some of this destruction in mice was significantly improved, including an increase in the bone volume fraction (BV\/TV) and bone trabecular number (Tb. N), decrease in bone separation (Tb. SP), reduction in the damage to the mouse cartilage layer, reduction in osteoclast lacunae in bone trabecula, and reduction in the damage to the bone dense part. We also found that the expression of ALP, \u03b2\u2010Catenin, Runx2, Osterix, and bone morphogenetic protein (BMP)2 significantly decreased in OVX mice but increased after TDN treatment. Therefore, this study suggests that TDNs may regulate the Wnt\/\u03b2\u2010Catenin and BMP signalling pathways to improve the levels of some specific markers of osteogenic differentiation, such as Runx2, ALP, and Osterix, to promote osteogenesis, thus showing a therapeutic effect on OP mice.<\/jats:p>","DOI":"10.1111\/cpr.13625","type":"journal-article","created":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T06:30:45Z","timestamp":1709101845000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Effects of tetrahedral <scp>DNA<\/scp> nanostructures on the treatment of osteoporosis"],"prefix":"10.1111","volume":"57","author":[{"given":"Weitong","family":"Cui","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Yang","sequence":"additional","affiliation":[{"name":"Psychiatric Laboratory and Mental Health Center, the State Key Laboratory of Biotherapy West China Hospital of Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6210-3206","authenticated-orcid":false,"given":"Yikai","family":"Dou","sequence":"additional","affiliation":[{"name":"Psychiatric Laboratory and Mental Health Center, the State Key Laboratory of Biotherapy West China Hospital of Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Du","sequence":"additional","affiliation":[{"name":"Psychiatric Laboratory and Mental Health Center, the State Key Laboratory of Biotherapy West China Hospital of Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohong","family":"Ma","sequence":"additional","affiliation":[{"name":"Psychiatric Laboratory and Mental Health Center, the State Key Laboratory of Biotherapy West China Hospital of Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Sichuan Langsheng Brain Hospital &amp; Shanghai Langsheng Brain Hospital Investment Co., Ltd.  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1224-6561","authenticated-orcid":false,"given":"Yunfeng","family":"Lin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology Sichuan University  Chengdu China"},{"name":"Sichuan Provincial Engineering Research Center of Oral Biomaterials  Chengdu China"},{"name":"National Center for Translational Medicine Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,2,27]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"435","article-title":"Osteoporosis \u2013 a current view of pharmacological prevention and treatment","volume":"7","author":"Das S","year":"2013","journal-title":"Drug des Devel Ther"},{"issue":"21","key":"e_1_2_9_3_1","first-page":"2096","article-title":"Postmenopausal osteoporosis","volume":"374","author":"Cheung A","year":"2016","journal-title":"N Engl J Med"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.2072"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI27071"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-018-2981-y"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.21037\/apm-22-998"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.0c15133"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2022.116610"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/17455057221125577"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.55.9.693"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/burnst\/tkac006"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-021-00727-9"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202107820"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cclet.2021.11.090"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-020-0355-z"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41413-019-0077-4"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nano.2017.02.011"},{"issue":"2","key":"e_1_2_9_19_1","first-page":"164","article-title":"Animal models for bone and joint disease. 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