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However, its function in porcine embryonic development is unknown. In this study, we used an NEK2\u2010specific inhibitor, JH295 (JH), to investigate the role of NEK2 in embryonic development and the underlying regulatory mechanisms. Inhibition of NEK2 after parthenogenesis activation or in vitro fertilization significantly reduced the rates of cleavage and blastocyst formation, the numbers of trophectoderm and total cells and the cellular survival rate compared with the control condition. NEK2 inhibition delayed cell cycle progression at all stages from interphase to cytokinesis during the first mitotic division; it caused abnormal nuclear morphology in two\u2010 and four\u2010cell stage embryos. Additionally, NEK2 inhibition significantly increased DNA damage and apoptosis, and it altered the expression levels of DNA damage repair\u2010 and apoptosis\u2010related genes. Intriguingly, NEK2 inhibition downregulated the expression of \u03b2\u2010catenin and its downstream target genes. To validate the relationship between Wnt\/\u03b2\u2010catenin signalling and NEK2 during porcine embryonic development, we cultured porcine embryos in JH\u2010treated medium with or without CHIR99021, a Wnt activator. CHIR99021 co\u2010treatment strongly restored the developmental parameters reduced by NEK2 inhibition to control levels. Our findings suggest that NEK2 plays an essential role in porcine embryonic development by regulating DNA damage repair and normal mitotic division via the Wnt\/\u03b2\u2010catenin signalling pathway.<\/jats:p>","DOI":"10.1111\/cpr.13626","type":"journal-article","created":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T08:49:13Z","timestamp":1709282953000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["<scp>NEK2<\/scp> plays an essential role in porcine embryonic development by maintaining mitotic division and <scp>DNA<\/scp> damage response via the Wnt\/\u03b2\u2010catenin signalling pathway"],"prefix":"10.1111","volume":"57","author":[{"given":"Se\u2010Been","family":"Jeon","sequence":"first","affiliation":[{"name":"Futuristic Animal Resource &amp; Research Center (FARRC) Korea Research Institute of Bioscience and Biotechnology (KRIBB)  Cheongju Republic of Korea"},{"name":"Department of Animal Science, College of Natural Resources &amp; Life Science Pusan National University  Miryang Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pil\u2010Soo","family":"Jeong","sequence":"additional","affiliation":[{"name":"Futuristic Animal Resource &amp; Research Center (FARRC) Korea Research Institute of Bioscience and Biotechnology (KRIBB)  Cheongju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyo\u2010Gu","family":"Kang","sequence":"additional","affiliation":[{"name":"Futuristic Animal Resource &amp; Research Center (FARRC) Korea Research Institute of Bioscience and Biotechnology (KRIBB)  Cheongju Republic of Korea"},{"name":"Department of Animal Science and Biotechnology, College of Agriculture and Life Science Chungnam National University  Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min Ju","family":"Kim","sequence":"additional","affiliation":[{"name":"Futuristic Animal 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