{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T20:13:20Z","timestamp":1791922400638,"version":"4.3.4"},"reference-count":98,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T00:00:00Z","timestamp":1720569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31471384"],"award-info":[{"award-number":["31471384"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31972893"],"award-info":[{"award-number":["31972893"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32370841"],"award-info":[{"award-number":["32370841"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["92054109"],"award-info":[{"award-number":["92054109"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A specialised microenvironment, termed niche, provides extrinsic signals for the maintenance of residential stem cells. However, how residential stem cells maintain niche homeostasis and whether stromal niche cells could convert their fate into stem cells to replenish lost stem cells upon systemic stem cell loss remain largely unknown. Here, through systemic identification of JAK\/STAT downstream targets in adult <jats:italic>Drosophila<\/jats:italic> testis, we show that Escargot (Esg), a member of the Snail family of transcriptional factors, is a putative JAK\/STAT downstream target. <jats:italic>esg<\/jats:italic> is intrinsically required in cyst stem cells (CySCs) but not in germline stem cells (GSCs). <jats:italic>esg<\/jats:italic> depletion in CySCs results in CySC loss due to differentiation and non\u2010cell autonomous GSC loss. Interestingly, hub cells are gradually lost by delaminating from the hub and converting into CySCs in <jats:italic>esg<\/jats:italic>\u2010<jats:italic>defective<\/jats:italic> testes. Mechanistically, <jats:italic>esg<\/jats:italic> directly represses the expression of <jats:italic>socs36E<\/jats:italic>, the well\u2010known downstream target and negative regulator of JAK\/STAT signalling. Finally, further depletion of <jats:italic>socs36E<\/jats:italic> completely rescues the defects observed in <jats:italic>esg<\/jats:italic>\u2010<jats:italic>defective<\/jats:italic> testes. Collectively, JAK\/STAT target Esg suppresses SOCS36E to maintain CySC fate and repress niche cell conversion. Thus, our work uncovers a regulatory loop between JAK\/STAT signalling and its downstream targets in controlling testicular niche homeostasis under physiological conditions.<\/jats:p>","DOI":"10.1111\/cpr.13648","type":"journal-article","created":{"date-parts":[[2024,7,11]],"date-time":"2024-07-11T03:39:11Z","timestamp":1720669151000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A regulatory loop of <scp>JAK<\/scp>\/<scp>STAT<\/scp> signalling and its downstream targets represses cell fate conversion and maintains male germline stem cell niche homeostasis"],"prefix":"10.1111","volume":"57","author":[{"given":"Ruiyan","family":"Kong","sequence":"first","affiliation":[{"name":"Laboratory of Stem Cell Biology, College of Life Sciences Capital Normal University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Laboratory of Stem Cell Biology, College of Life Sciences Capital Normal University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory of Stem Cell Biology, College of Life Sciences Capital Normal University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yankun","family":"Ma","sequence":"additional","affiliation":[{"name":"Laboratory of Stem Cell Biology, College of Life Sciences Capital Normal University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ningfang","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory of Stem Cell Biology, College of Life Sciences Capital Normal University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Shi","sequence":"additional","affiliation":[{"name":"Laboratory of Stem Cell Biology, College of Life Sciences Capital Normal University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7660-8579","authenticated-orcid":false,"given":"Zhouhua","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory of Stem Cell Biology, College of Life Sciences Capital Normal University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,10]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"7","article-title":"The relationship between the spleen colony\u2010forming cell and the haemopoietic stem cell","volume":"4","author":"Schofield R","year":"1978","journal-title":"Blood Cells"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-022-00510-w"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2022.07.003"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2010.02.010"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2012.01.004"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.290.5490.328"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00255-7"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.ctdb.2015.11.041"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1110542"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.01.038"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04957"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.17.1.387"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.002022"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.08.083"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07173"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2014.04.025"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1474-9726.2006.00221.x"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2007.08.002"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.201148"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.70810"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2014.03.058"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.122.8.2437"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1140861"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-100913-013344"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(79)90108-4"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1101\/sqb.2008.73.023"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2005.08.012"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1066707"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2008.05.001"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1066700"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1087795"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.24.5463"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.057901"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.056242"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.05.037"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1176817"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.82201"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2006.02.030"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.057364"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2021.07.010"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01025"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.086413"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.075242"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1426"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2013.29"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2003.10.063"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2011.06.022"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.4161\/jkst.21621"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.167643"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2018.04.011"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2023.08.007"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200711022"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-42630-y"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2010.02.006"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2086"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1205618"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2121-5-38"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02411"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.22073"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e13-05-0275"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2006.02.048"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1005815"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/74487"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2009.309"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.139261"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/0925-4773(92)90063-P"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm757"},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.1038\/35000034"},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.1038\/35000025"},{"key":"e_1_2_8_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.02.008"},{"key":"e_1_2_8_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.03.027"},{"key":"e_1_2_8_73_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01907"},{"key":"e_1_2_8_74_1","doi-asserted-by":"publisher","DOI":"10.4161\/cam.4.2.10943"},{"key":"e_1_2_8_75_1","doi-asserted-by":"publisher","DOI":"10.2174\/15680096113136660102"},{"key":"e_1_2_8_76_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.122.12.3697"},{"key":"e_1_2_8_77_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201489072"},{"key":"e_1_2_8_78_1","doi-asserted-by":"publisher","DOI":"10.1080\/19336934.2016.1176650"},{"key":"e_1_2_8_79_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201489050"},{"key":"e_1_2_8_80_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.133322"},{"key":"e_1_2_8_81_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2022.110679"},{"key":"e_1_2_8_82_1","doi-asserted-by":"publisher","DOI":"10.1038\/35037606"},{"key":"e_1_2_8_83_1","first-page":"159","article-title":"Mgm 1, the earliest sex\u2010specific germline marker in Drosophila, reflects expression of the gene esg in male stem cells","volume":"46","author":"Streit A","year":"2002","journal-title":"Int J Dev Biol"},{"key":"e_1_2_8_84_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb07285.x"},{"key":"e_1_2_8_85_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(00)01791-4"},{"key":"e_1_2_8_86_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1058"},{"key":"e_1_2_8_87_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0012-1606(03)00127-1"},{"key":"e_1_2_8_88_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.149807"},{"key":"e_1_2_8_89_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2022.07.014"},{"key":"e_1_2_8_90_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(02)00216-2"},{"key":"e_1_2_8_91_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2012.12.019"},{"key":"e_1_2_8_92_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2008.07.010"},{"key":"e_1_2_8_93_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.139782"},{"key":"e_1_2_8_94_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004713"},{"key":"e_1_2_8_95_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201490881"},{"key":"e_1_2_8_96_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201387500"},{"key":"e_1_2_8_97_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.11.014"},{"key":"e_1_2_8_98_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.192021.112"},{"key":"e_1_2_8_99_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1009609"}],"container-title":["Cell Proliferation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cpr.13648","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T03:38:55Z","timestamp":1728877135000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cpr.13648"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,10]]},"references-count":98,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["10.1111\/cpr.13648"],"URL":"https:\/\/doi.org\/10.1111\/cpr.13648","archive":["Portico"],"relation":{},"ISSN":["0960-7722","1365-2184"],"issn-type":[{"value":"0960-7722","type":"print"},{"value":"1365-2184","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,10]]},"assertion":[{"value":"2024-01-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13648"}}