{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T18:51:11Z","timestamp":1792176671228,"version":"4.4.0"},"reference-count":50,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,6,10]],"date-time":"2024-06-10T00:00:00Z","timestamp":1717977600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In vitro T\u2010cell differentiation from pluripotent stem cells (PSCs) could potentially provide an unlimited source of T cells for cancer immunotherapy, which, however is still hindered by the inefficient obtaining functionally\u2010matured, terminally\u2010differentiated T cells. Here, we established a fluorescence reporter human induced pluripotent stem cell (iPSC) line termed TCF7<jats:sup>mCherry<\/jats:sup>RUNX1<jats:sup>GFP<\/jats:sup>, in which the endogenous expression of RUNX1 and TCF7 are illustrated by the GFP and mCherry fluorescence, respectively. Utilizing TCF7<jats:sup>mCherry<\/jats:sup>RUNX1<jats:sup>GFP<\/jats:sup>, we defined that the feeder cells incorporating CXCL12\u2010expressing OP9 cells with DL4\u2010expressing OP9 cells at a 1:3 ratio (OP9\u2010C1D3) significantly enhanced efficiency of CD8<jats:sup>+<\/jats:sup> T cell differentiation from PSCs. Additionally, we engineered a chimeric antigen receptor (CAR) targeting EGFR into iPSCs. The CAR\u2010T cells differentiated from these iPSCs using OP9\u2010C1D3 feeders demonstrated effective cytotoxicity toward lung cancer cells. We anticipate this platform will help the in vitro HSPC and T cell differentiation optimization, serving the clinical demands of these cells.<\/jats:p>","DOI":"10.1111\/cpr.13661","type":"journal-article","created":{"date-parts":[[2024,6,10]],"date-time":"2024-06-10T08:59:07Z","timestamp":1718009947000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Optimizing in vitro T cell differentiation by using induced pluripotent stem cells with <scp>GFP\u2010RUNX1<\/scp> and <scp>mCherry\u2010TCF7<\/scp> labelling"],"prefix":"10.1111","volume":"57","author":[{"given":"Yu","family":"Zhao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Stem Cell and Reproductive Biology Institute for Stem Cell and Regeneration, Institute of Zoology, Chinese Academy of Sciences  Beijing China"},{"name":"University of Chinese Academy of Sciences  Beijing 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Reproductive Biology Institute for Stem Cell and Regeneration, Institute of Zoology, Chinese Academy of Sciences  Beijing China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenxi","family":"Cheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Stem Cell and Reproductive Biology Institute for Stem Cell and Regeneration, Institute of Zoology, Chinese Academy of Sciences  Beijing China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaojing","family":"Tan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Stem Cell and Reproductive Biology Institute for Stem Cell and Regeneration, Institute of Zoology, Chinese Academy of Sciences  Beijing China"},{"name":"University of Chinese Academy of Sciences  Beijing 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