{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T12:20:20Z","timestamp":1790079620951,"version":"4.0.1"},"reference-count":40,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T00:00:00Z","timestamp":1719964800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFA0915000"],"award-info":[{"award-number":["2023YFA0915000"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82171102"],"award-info":[{"award-number":["82171102"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dry eye disease (DED) is a growing public health concern affecting millions of people worldwide and causing ocular discomfort and visual disturbance. Developing its therapeutic drugs based on animal models suffer from interspecies differences and poor prediction of human trials. Here, we established long\u2010term 3D human corneal epithelial organoids, which recapitulated the cell lineages and gene expression signature of the human corneal epithelium. Organoids can be regulated to differentiate ex vivo, but the addition of FGF10 inhibits this process. In the hyperosmolar\u2010induced DED organoid model, the release of inflammatory factors increased, resulting in damage to the stemness of stem cells and a decrease in functional mucin 1 protein. Furthermore, we found that the organoids could mimic clinical drug treatment responses, suggesting that corneal epithelial organoids are promising candidates for establishing a drug testing platform ex vivo. In summary, we established a functional, long\u2010term 3D human epithelial organoid that may serve as an ex vivo model for studying the functional regulation and disease modelling.<\/jats:p>","DOI":"10.1111\/cpr.13704","type":"journal-article","created":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T05:03:55Z","timestamp":1720069435000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Establishment of human corneal epithelial organoids for ex vivo modelling dry eye disease"],"prefix":"10.1111","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4550-0178","authenticated-orcid":false,"given":"Xichen","family":"Wan","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Eye &amp; ENT Hospital, State Key Laboratory of Molecular Engineering of Polymers Fudan University  Shanghai China"},{"name":"NHC Key laboratory of Myopia and Related Eye Diseases  Shanghai China"},{"name":"Shanghai Engineering Research Center of Synthetic Immunology  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayu","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Eye &amp; ENT Hospital, State Key Laboratory of Molecular Engineering of Polymers Fudan University  Shanghai China"},{"name":"NHC Key laboratory of Myopia and Related Eye Diseases  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9719-4889","authenticated-orcid":false,"given":"Xujiao","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Eye &amp; ENT Hospital, State Key Laboratory of Molecular Engineering of Polymers Fudan University  Shanghai China"},{"name":"NHC Key laboratory of Myopia and Related Eye Diseases  Shanghai China"},{"name":"Shanghai Engineering Research Center of Synthetic Immunology  Shanghai 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Engineering Macau University of Science and Technology  Taipa Macau SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxu","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Eye &amp; ENT Hospital, State Key Laboratory of Molecular Engineering of Polymers Fudan University  Shanghai China"},{"name":"NHC Key laboratory of Myopia and Related Eye Diseases  Shanghai China"},{"name":"Shanghai Engineering Research Center of Synthetic Immunology  Shanghai China"},{"name":"Department of Ophthalmology Children's Hospital of Fudan University, National Pediatric Medical Center of China  Shanghai 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