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Notably, Tmem184b exhibits high expression levels in the retina; however, its specific function within this tissue remains poorly understood. To elucidate the role of <jats:italic>Tmem184b<\/jats:italic> in the mammalian visual system, we developed a <jats:italic>Tmem184b<\/jats:italic> knockout (KO) model for further investigation. Loss of <jats:italic>Tmem184b<\/jats:italic> led to significant decreases in both a and b wave amplitudes of scotopic electroretinogram (ERG) and reduced b wave amplitudes of photopic ERG, respectively, reflecting damage to both the photoreceptors and secondary neuronal cells of the retina. Histologic analyses showed a progressive retinal thinning accompanied by the significantly loss of retinal cells including cone, rod, bipolar, horizontal and retinal ganglion cells. The expression levels of photo\u2010transduction\u2010related proteins were down\u2010regulated in KO retina. TUNEL (terminal deoxynucleotidyl transferase\u2010mediated biotinylated Uridine\u20105'\u2010triphosphate [UTP]\u00a0nick end labelling) and glial fibrillary acidic protein (GFAP)\u2010labelling results suggested the increased cell death and inflammation in the KO mice. RNA\u2010sequencing analysis and GO enrichment analysis revealed that <jats:italic>Tmem184b<\/jats:italic> deletion resulted in down\u2010regulated genes involved in various biological processes such as visual perception, response to hypoxia, regulation of transmembrane transporter activity. Taken together, our study revealed essential roles of <jats:italic>Tmem184b<\/jats:italic> in the mammalian retina and confirmed the underlying mechanisms including cell death, inflammation and hypoxia pathway in the absence of <jats:italic>Tmem184b<\/jats:italic>, providing a potential target for therapeutic and diagnostic development.<\/jats:p>","DOI":"10.1111\/cpr.13751","type":"journal-article","created":{"date-parts":[[2024,10,8]],"date-time":"2024-10-08T05:46:22Z","timestamp":1728366382000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Deletion of <i>Transmembrane protein 184b<\/i> leads to retina degeneration in mice"],"prefix":"10.1111","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5596-4227","authenticated-orcid":false,"given":"Guo","family":"Liu","sequence":"first","affiliation":[{"name":"The Sichuan Provincial Key Laboratory for Human Disease Gene Study and Center for Medical 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