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Single\u2010nucleus RNA sequencing (snRNA\u2010seq) was used to reveal the transcriptional changes in the supraspinatus muscle after RCT. Supraspinatus muscles were obtained from patients with habitual shoulder dislocation (<jats:italic>n<\/jats:italic>\u2009=\u20093) and RCT (<jats:italic>n<\/jats:italic>\u2009=\u20093). In response to the RCT, trajectory analysis showed progression from normal myonuclei to ANKRD1<jats:sup>+<\/jats:sup> myonuclei, which captured atrophy\u2010and fatty infiltration\u2010related regulons (KLF5, KLF10, FOSL1 and BHLHE40). Transcriptomic alterations in fibro\/adipogenic progenitors (FAPs) and muscle satellite cells (MuSCs) have also been studied. By predicting cell\u2013cell interactions, we observed communication alterations between myofibers and muscle\u2010resident cells following RCT. Our findings reveal the plasticity of muscle cells in response to RCT and offer valuable insights into the molecular mechanisms and potential therapeutic targets of RCT.<\/jats:p>","DOI":"10.1111\/cpr.13763","type":"journal-article","created":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T09:42:56Z","timestamp":1729590176000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Single\u2010nucleus transcriptomics reveals subsets of degenerative myonuclei after rotator cuff tear\u2010induced muscle atrophy"],"prefix":"10.1111","volume":"58","author":[{"given":"Ziying","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Orthopedics, Nanjing Jinling Hospital, Affiliated Hospital of Medical School Nanjing University  Nanjing Jiangsu People's Republic of 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