{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T13:49:07Z","timestamp":1793022547848,"version":"4.5.0"},"reference-count":44,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T00:00:00Z","timestamp":1742169600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Human periodontal ligament stem cells (hPDLSCs) have emerged as promising candidates for the treatment of osteoporotic bone defects. Previous studies have indicated that m<jats:sup>6<\/jats:sup>A plays a crucial role in regulating the osteogenic differentiation of hPDLSCs. However, research on the relationship between YTHDC1, as a reading protein, and the osteogenic differentiation of hPDLSCs remains unexplored. This study aimed to investigate the biological roles of YTHDC1 in the osteogenic differentiation of hPDLSCs and to explore underlying mechanisms. Dot blot analysis revealed a progressive increase in m<jats:sup>6<\/jats:sup>A methylation during osteogenic differentiation, accompanied by significant upregulation of YTHDC1 expression, as evidenced by qPCR and Western blot. Functional assays utilising siRNA\u2010mediated knockdown and lentiviral\u2010mediated overexpression demonstrated that YTHDC1 positively regulated the osteogenic differentiation potential of hPDLSCs. Mechanistically, mRNA\u2010seq analysis implicated the Wnt\/\u03b2\u2010catenin signalling pathway, which was further validated through rescue experiments with the Wnt inhibitor DKK1. Notably, in\u00a0vivo experiments showed that hPDLSCs overexpressing <jats:italic>YTHDC1<\/jats:italic> exhibited enhanced bone formation capacity in the osteoporotic rats. In conclusion, our findings suggested that YTHDC1 modulated the osteogenic capacity of hPDLSCs through the Wnt\/\u03b2\u2010catenin signalling pathway, highlighting its therapeutic potential for treating bone defects in osteoporotic conditions.<\/jats:p>","DOI":"10.1111\/cpr.70020","type":"journal-article","created":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T00:14:51Z","timestamp":1742256891000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["<scp>YTHDC1<\/scp> Modulates the Osteogenic Capacity of <scp>hPDLSCs<\/scp> via Wnt\/\u03b2\u2010Catenin Signalling Pathway for the Treatment of Bone Defects in Osteoporosis Rats"],"prefix":"10.1111","volume":"58","author":[{"given":"Dan","family":"Tan","sequence":"first","affiliation":[{"name":"Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education College of 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