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Follicular fluid (FF) serves as the microenvironment for follicular development and oocyte maturation, gaining an in\u2010depth understanding of the metabolic state of FF will help us uncover the key biological processes involved in ovarian aging, while the specific underlying pathogenic mechanisms are not fully understood. In this study, we utilised pseudotargeted metabolomic analysis of FF to reveal the glycerophospholipid metabolism dysfunction mediated by <jats:italic>GPD1L<\/jats:italic> in DOR patients. We also found that <jats:italic>GPD1L<\/jats:italic> was downregulated in granulosa cells (GCs) of DOR patients, resulting in increased cell apoptosis and mitochondrial dysfunction. Moreover, our results demonstrated that the downregulated expression of <jats:italic>GPD1L<\/jats:italic> could induce follicular atresia and impair oocyte quality in mouse ovaries. Altogether, our research suggested that <jats:italic>GPD1L<\/jats:italic> in GCs and the key metabolites in the glycerophospholipid metabolism pathway could potentially act as novel biomarkers of DOR diagnosis, paving the way for a new theoretical basis for understanding the pathogenesis of DOR.<\/jats:p>","DOI":"10.1111\/cpr.70024","type":"journal-article","created":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T03:31:18Z","timestamp":1742441478000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["<scp>GPD1L<\/scp>\u2010Mediated Glycerophospholipid Metabolism Dysfunction in Women With Diminished Ovarian Reserve: Insights From Pseudotargeted Metabolomic Analysis of Follicular Fluid"],"prefix":"10.1111","volume":"58","author":[{"given":"Jiaqi","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Human Reproductive Medicine, Beijing Obstetrics and Gynecology Hospital, Beijing Maternal and Child 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