{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T22:20:36Z","timestamp":1789510836038,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T00:00:00Z","timestamp":1749772800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2024JJ5494"],"award-info":[{"award-number":["2024JJ5494"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>With the continuous increase of the elderly population and the deepening of population ageing in China, osteoporosis has gradually become one of the significant public health problems. Elucidating the pathophysiological mechanisms that induce osteoporosis and identifying more effective therapeutic targets is of great clinical significance. In this study, in\u00a0vitro experiments demonstrated that endothelial cell exosomes (EC\u2010EXOs) promoted osteogenic and inhibited adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). Aged and ovariectomy (OVX)\u2010induced osteoporosis mice models injected with EC\u2010EXOs confirmed that EC\u2010EXOs delayed bone loss. Proteomic analysis revealed a key protein regulating the differentiation of BMSCs. Expression of THBS3 was significantly higher in EC\u2010EXOs than in Human microvascular endothelial cells (HMEC\u20101). In\u00a0vitro and in\u00a0vivo experiments further validated that THBS3 promoted BMSCs' osteogenic differentiation, inhibited their adipogenic differentiation, and retarded bone loss. Computational biology analysis found that CD47 is a downstream target and potentially functional receptor in BMSCs that bind to THBS3. THBS3 treatment of BMSCs down\u2010regulated the expression of CD47 in in\u00a0vitro experiments. The aged\/OVX models further confirmed that EC\u2010EXOs can regulate the differentiation of BMSCs and delay the process of bone loss via the THBS3\u2013CD47 axis. CD47 antibody may be a potential therapeutic agent for treating ageing\u2010associated bone loss.<\/jats:p>","DOI":"10.1111\/cpr.70066","type":"journal-article","created":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T22:46:58Z","timestamp":1749854818000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Mechanism of\n                    <scp>EC<\/scp>\n                    \u2010\n                    <scp>EXOs<\/scp>\n                    \u2010Derived\n                    <scp>THBS3<\/scp>\n                    Targeting\n                    <scp>CD47<\/scp>\n                    to Regulate\n                    <scp>BMSCs<\/scp>\n                    Differentiation to Ameliorate Bone Loss"],"prefix":"10.1111","volume":"58","author":[{"given":"Jiaojiao","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pharmacy The Second Xiangya Hospital, Central South University  Changsha Hunan People's 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