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Here, we found that the SNARE family member, YKT6, is indispensable for male fertility in mice. Conditional\n                    <jats:italic>Ykt6<\/jats:italic>\n                    knockout in pre\u2010meiotic and meiotic germ cells leads to complete sterility and meiotic arrest in male mice, which exhibit loss of spermatocytes in seminiferous tubules, but without obvious disruption of chromosomal behaviours during meiosis. We observed that the abundance of syncytia increases along with abnormal morphology of the Golgi apparatus, while lysosomes decrease in\n                    <jats:italic>Ykt6<\/jats:italic>\n                    \u2010cKO testes. Quantitative proteomics and immunofluorescent staining both showed dysregulation of vesicular transport in YKT6\u2010deficient spermatocytes. Additionally, the recombinant mouse proteins, HA::YKT6 and MYC::STX1A, could interact in\u00a0vitro, further supporting a likely role in mediating transport vesicle fusion with the plasma membrane. Finally, the absence of TEX14 signal within syncytia and enlarged TEX14 rings between spermatocytes together suggest a failure to stabilise intercellular bridges in\n                    <jats:italic>Ykt6<\/jats:italic>\n                    \u2010cKO testes. These results demonstrate that YKT6 is required for male fertility by promoting meiosis progression through vesicular transport regulation during spermatogenesis in mice, expanding our understanding of YKT6 functions, and suggesting a possible strategy for future interventions for male infertility in humans.\n                  <\/jats:p>","DOI":"10.1111\/cpr.70079","type":"journal-article","created":{"date-parts":[[2025,6,29]],"date-time":"2025-06-29T10:27:43Z","timestamp":1751192863000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>YKT6<\/scp>\n                    Is Essential for Male Fertility by Promoting Meiosis Progression During Spermatogenesis of Mice"],"prefix":"10.1111","volume":"59","author":[{"given":"Jie","family":"Cen","sequence":"first","affiliation":[{"name":"Institute of Women, Children and Reproductive Health Shandong University  China"},{"name":"State Key Laboratory of Reproductive 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Reproductive Health Shandong University  China"},{"name":"State Key Laboratory of Reproductive Medicine and Offspring Health Shandong University  Jinan Shandong China"},{"name":"National Research Center for Assisted Reproductive Technology and Reproductive Genetics Shandong University  Jinan Shandong China"},{"name":"Key Laboratory of Reproductive Endocrinology (Shandong University) Ministry of Education  Jinan Shandong China"},{"name":"Shandong Technology Innovation Center for Reproductive Health  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4029-6411","authenticated-orcid":false,"given":"Fei","family":"Gao","sequence":"additional","affiliation":[{"name":"Institute of Women, Children and Reproductive Health Shandong University  China"},{"name":"State Key Laboratory of Reproductive Medicine and Offspring Health Shandong University  Jinan Shandong China"},{"name":"National Research Center for Assisted Reproductive 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