{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T02:22:15Z","timestamp":1790130135449,"version":"4.0.1"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T00:00:00Z","timestamp":1751587200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T00:00:00Z","timestamp":1751587200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82270812"],"award-info":[{"award-number":["82270812"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["823B2013"],"award-info":[{"award-number":["823B2013"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Benign prostatic hyperplasia (BPH) is a common condition in older men, with its prevalence increasing as age advances. Chronic inflammation orchestrates oxidative stress to exacerbate BPH. YAP1, which regulates organ size, cellular homeostasis, and tissue fibrosis, can be activated by ROCK1. Given the urgent clinical need for more effective therapies, this study explored whether targeting the ROCK1\/YAP1 axis could mitigate BPH progression. Here, rats received in\u00a0situ adeno\u2010associated virus (AAV) injection to induce prostate\u2010specific YAP1 overexpression. An inflammation\u2010associated experimental autoimmune prostatitis (EAP) model was established by prostate antigen immunisation, followed by treatment with ROCK1 inhibitor fasudil and YAP1 inhibitor verteporfin. Cell models were treated with specific inhibitors to confirm the critical role of YAP1 in modulating mitochondrial function. As a result, YAP1 overexpression was sufficient to induce a pathological BPH phenotype. Specifically, YAP1 activated the inflammatory cascade to provoke an immune response, disrupted proliferation\/apoptosis balance to induce tissue hyperplasia, triggered epithelial\u2010mesenchymal transition (EMT) and reactive stroma to drive fibrosis, and promoted NOX4\/ROS generation and antioxidant depletion to cause oxidative stress. The inflammation\u2010induced experimental autoimmune prostatitis (EAP) model also presented analogous BPH lesions, which were significantly alleviated when treated with ROCK1 inhibitor fasudil and YAP1 inhibitor verteporfin. Mechanistically, YAP1 activation under inflammatory conditions suppressed SIRT1 expression, thereby exacerbating oxidative stress through the disruption of DRP1\/MFN2\u2010mediated mitochondrial dynamics. Overall, inflammation\u2010driven activation of the ROCK1\/YAP1 axis aggravates oxidative stress, promoting BPH hyperplasia and fibrosis by impairing SIRT1\u2010regulated mitochondrial dynamics. These findings provide a preclinical rationale for developing ROCK1 or YAP1 inhibitors as targeted therapies for BPH patients with chronic inflammation.<\/jats:p>","DOI":"10.1111\/cpr.70085","type":"journal-article","created":{"date-parts":[[2025,7,5]],"date-time":"2025-07-05T01:33:33Z","timestamp":1751679213000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Targeting\n                    <scp>ROCK1<\/scp>\n                    \/\n                    <scp>YAP1<\/scp>\n                    Axis Ameliorates Inflammation\u2010Induced Prostatic Hyperplasia via Stabilising\n                    <scp>SIRT1<\/scp>\n                    \u2010Dependent Mitochondrial Dynamics"],"prefix":"10.1111","volume":"59","author":[{"given":"Dongxu","family":"Lin","sequence":"first","affiliation":[{"name":"Department and Institute of Urology Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengyu","family":"Wei","sequence":"additional","affiliation":[{"name":"Department and Institute of Urology Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengyang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang","family":"Li","sequence":"additional","affiliation":[{"name":"Department and Institute of Urology Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina","family":"Li","sequence":"additional","affiliation":[{"name":"Department and Institute of Urology Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhipeng","family":"Li","sequence":"additional","affiliation":[{"name":"Department and Institute of Urology Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changcheng","family":"Luo","sequence":"additional","affiliation":[{"name":"Department and Institute of Urology Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbo","family":"Kuang","sequence":"additional","affiliation":[{"name":"Department and Institute of Urology Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology  Wuhan Hubei 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