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Nevertheless, the temporal progression of post\u2010TBI neurogenesis and its molecular mechanisms remain elusive. To investigate the mechanism of impaired hippocampal neurogenesis and neurological deficits following TBI. Single\u2010cell RNA sequencing (scRNA\u2010seq) was employed to explore the mechanism of abnormal hippocampal neurogenesis after TBI in mice. Antagonists and conditional gene knockout (CKO) strategies were applied to dissect the molecular function of target genes. Here, we found that neural stem cells (NSCs) were hyperactivated as observed in Nestin\u2010GFP reporter mice in hippocampus during the early phases of TBI, followed by progressive depletion of the NSC pool, impaired neurogenesis, and the onset of progressive cognitive dysfunction. ScRNA\u2010seq transcriptomic analysis revealed sustained upregulation of Rho\u2010associated coiled\u2010coil protein kinase 1 (ROCK1) in hippocampal NSCs post\u2010TBI. Pharmacological inhibition of ROCK1 or ROCK1 CKO rescued chronic neurogenic deficits and improved cognitive functions in TBI mice. Mechanistically, ROCK1 dysregulation impaired neurogenesis via aberrant AKT hyperphosphorylation, establishing a unidirectional ROCK1\u2010AKT signalling axis in adult hippocampal neurogenesis. Our findings position ROCK1 as a pivotal regulator of the post\u2010TBI NSC pool hyperactivation and aberrant neurogenesis and propose targeted kinase inhibition strategies as a potential therapy to mitigate abnormal neurogenesis in TBI patients.<\/jats:p>","DOI":"10.1111\/cpr.70093","type":"journal-article","created":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T23:38:40Z","timestamp":1754091520000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Dysregulation of Rho\u2010Associated Coiled\u2010Coil Protein Kinase1 Depletes Neural Stem Cell Pool and Impairs Hippocampal Neurogenesis After Traumatic Brain Injury"],"prefix":"10.1111","volume":"59","author":[{"given":"Chaoqun","family":"Yao","sequence":"first","affiliation":[{"name":"Department of Neurosurgery and Key Laboratory of Neurotrauma, Southwest Hospital Third Military Medical University (Army Medical University)  Chongqing China"},{"name":"Department of 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