{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T01:51:27Z","timestamp":1777945887650,"version":"3.51.4"},"reference-count":53,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T00:00:00Z","timestamp":1762128000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T00:00:00Z","timestamp":1762128000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82000997"],"award-info":[{"award-number":["82000997"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Circadian rhythm is an essential biological process that synchronises physiological activities with environmental light\/dark cycles. However, its regulatory mechanisms in tooth development remain incompletely understood. Here, we investigated the role of the\n                    <jats:italic>p75<\/jats:italic>\n                    neurotrophin receptor (\n                    <jats:italic>p75NTR<\/jats:italic>\n                    ) in circadian rhythm regulation and daily mineralization during tooth development using immunofluorescence, circadian rhythm tracking, and genetic models. Spatiotemporal analysis of rat dental germs revealed that oscillatory expression patterns of\n                    <jats:italic>p75NTR<\/jats:italic>\n                    closely aligned with clock genes (\n                    <jats:italic>Bmal1<\/jats:italic>\n                    ,\n                    <jats:italic>Clock<\/jats:italic>\n                    ,\n                    <jats:italic>Per1<\/jats:italic>\n                    ,\n                    <jats:italic>Per2<\/jats:italic>\n                    ), mineralization\u2010related factors, and odontogenesis\u2010related factors.\n                    <jats:italic>p75NTR<\/jats:italic>\n                    knockout mice (\n                    <jats:italic>p75NTR<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>ExIII\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    ) exhibited reduced body weight, lower melatonin levels, delayed incisor eruption, decreased daily mineralization width, and downregulation of core clock genes. Mechanistically,\n                    <jats:italic>p75NTR<\/jats:italic>\n                    overexpression in immortalised stem cells from the dental apical papilla (iSCAPs) upregulated casein kinase 2 (CK2) expression, enhanced PER2 phosphorylation, and promoted nuclear p\u2010PER2 accumulation, while CK2 inhibition partially reversed these effects. In\u00a0vivo, CK2 inhibition via quinalizarin exacerbated incisor eruption defects in\n                    <jats:italic>p75NTR<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>ExIII\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    mice. These findings demonstrate that\n                    <jats:italic>p75NTR<\/jats:italic>\n                    regulates circadian\u2010driven mineralization and tooth morphogenesis, probably via the CK2\/PER2 pathway, providing critical insight into the interplay between the circadian rhythm and dental development.\n                  <\/jats:p>","DOI":"10.1111\/cpr.70140","type":"journal-article","created":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T07:02:17Z","timestamp":1762239737000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>p75NTR<\/i>\n                    Promotes Circadian\u2010Driven Mineralization During Tooth Development via\n                    <scp>CK2<\/scp>\n                    \/\n                    <scp>PER2<\/scp>\n                    Pathway"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-6779-5000","authenticated-orcid":false,"given":"Manzhu","family":"Zhao","sequence":"first","affiliation":[{"name":"College of Stomatology, Chongqing Medical University  Chongqing China"},{"name":"Chongqing Key Laboratory of Oral Diseases  Chongqing China"},{"name":"Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education  Chongqing China"},{"name":"Chongqing Municipal Health Commission Key Laboratory of Oral Biomedical Engineering  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongyan","family":"Yuan","sequence":"additional","affiliation":[{"name":"College of Stomatology, Chongqing Medical University  Chongqing China"},{"name":"Chongqing Key Laboratory of Oral Diseases  Chongqing China"},{"name":"Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education  Chongqing China"},{"name":"Chongqing Municipal Health Commission Key Laboratory of Oral Biomedical Engineering  Chongqing China"},{"name":"Department Orthodontics, Hospital of Stomatology Southwest Medical University  Luzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Di","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Stomatology, Chongqing Medical University  Chongqing China"},{"name":"Chongqing Key Laboratory of Oral Diseases  Chongqing China"},{"name":"Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education  Chongqing China"},{"name":"Chongqing Municipal Health Commission Key Laboratory of Oral Biomedical Engineering  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meng","family":"Li","sequence":"additional","affiliation":[{"name":"College of Stomatology, Chongqing Medical University  Chongqing China"},{"name":"Chongqing Key Laboratory of Oral Diseases  Chongqing China"},{"name":"Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education  Chongqing China"},{"name":"Chongqing Municipal Health Commission Key Laboratory of Oral Biomedical Engineering  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4355-0817","authenticated-orcid":false,"given":"Bo","family":"Xie","sequence":"additional","affiliation":[{"name":"College of Stomatology, Chongqing Medical University  Chongqing China"},{"name":"Chongqing Key Laboratory of Oral Diseases  Chongqing China"},{"name":"Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education  Chongqing China"},{"name":"Chongqing Municipal Health Commission Key Laboratory of Oral Biomedical Engineering  Chongqing China"},{"name":"Department Orthodontics, Hospital of Stomatology Southwest Medical University  Luzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuqiang","family":"Zou","sequence":"additional","affiliation":[{"name":"Department Orthodontics, Hospital of Stomatology Southwest Medical University  Luzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingjie","family":"Lu","sequence":"additional","affiliation":[{"name":"Department Orthodontics, Hospital of Stomatology Southwest Medical University  Luzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ye","family":"Qiu","sequence":"additional","affiliation":[{"name":"College of Stomatology, Chongqing Medical University  Chongqing China"},{"name":"Chongqing Key Laboratory of Oral Diseases  Chongqing China"},{"name":"Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education  Chongqing China"},{"name":"Chongqing Municipal Health Commission Key Laboratory of Oral Biomedical Engineering  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0224-6640","authenticated-orcid":false,"given":"Jinlin","family":"Song","sequence":"additional","affiliation":[{"name":"College of Stomatology, Chongqing Medical University  Chongqing China"},{"name":"Chongqing Key Laboratory of Oral Diseases  Chongqing China"},{"name":"Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education  Chongqing China"},{"name":"Chongqing Municipal Health Commission Key Laboratory of Oral Biomedical Engineering  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,3]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2022.875342"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smhs.2022.11.003"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41583-018-0026-z"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00009.2009"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aat4104"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1802337"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2013.01.003"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2022.1031519"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gep.2010.12.002"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41368-024-00317-9"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/0748730412442830"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41368-021-00123-7"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0159946"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/joa.13198"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1267\/ahc.19017"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2757-12.2013"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2022.981311"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2012.08.109"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-11555-9"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12800"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-27197-5"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2023.1283878"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12758"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00019.2022"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41574-021-00488-z"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202311659"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2024.02.008"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12865"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2022.110145"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-022-07292-6"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1080\/00016357.2016.1271999"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2013.02.011"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1042\/BC20060012"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2316858121"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2018.01.026"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202302029"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2024.114335"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2024.101078"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2020.10.061"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aau9060"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2018.09.002"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.abp8923"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2118286119"},{"key":"e_1_2_10_45_1","first-page":"273","article-title":"Nucleus\u2010Exported CLOCK Acetylates PRPS to Promote de Novo Nucleotide Synthesis and Liver Tumour Growth","volume":"25","author":"Liu T.","year":"2023","journal-title":"Nature Cell Biology"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2016.132"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-35795-8"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2018.06.013"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2023.05.045"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgg.2023.07.009"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.15390"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2019102374"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtm.2020.11.007"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2020.113608"}],"container-title":["Cell Proliferation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cpr.70140","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cpr.70140","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cpr.70140","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T13:47:10Z","timestamp":1777729630000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cpr.70140"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,3]]},"references-count":53,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1111\/cpr.70140"],"URL":"https:\/\/doi.org\/10.1111\/cpr.70140","archive":["Portico"],"relation":{},"ISSN":["0960-7722","1365-2184"],"issn-type":[{"value":"0960-7722","type":"print"},{"value":"1365-2184","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,3]]},"assertion":[{"value":"2025-07-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70140"}}