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Despite their ability to be maintained over multiple passages without losing stemness, conventional TSCs are characterised by notorious heterogeneity and a spontaneous differentiation tendency. In this study, we established a type of mouse TSCs with dome\u2010shaped colonies (dTSCs). The dTSCs expressed canonical markers of conventional TSCs and exhibited relatively low levels of heterogeneity and spontaneous differentiation when cultured in conventional trophoblast stem cell medium. Additionally, dTSCs retained robust potential to differentiate into all trophoblast lineages in\u00a0vitro, as well as the capacity to contribute to placental development in\u00a0vivo. Furthermore, the functions of\n                    <jats:italic>Foxo4<\/jats:italic>\n                    and\n                    <jats:italic>Slc16a3<\/jats:italic>\n                    (MCT4) could be effectively explored in trophoblast development by using our established dTSCs. Collectively, our findings establish a type of high\u2010quality TSCs and define dTSCs as a promising and refined model for investigating trophoblast development.\n                  <\/jats:p>","DOI":"10.1111\/cpr.70280","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T07:08:38Z","timestamp":1789715318000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Derivation of Mouse Dome\u2010Shaped Trophoblast Stem Cells With Low Heterogeneity From Blastocysts"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-5872-374X","authenticated-orcid":false,"given":"Minli","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Multi\u2010Organ Injury\u00a0Prevention and Treatment, Department of Developmental Biology, School of Basic Medical Sciences Southern Medical University  Guangzhou Guangdong 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School of Basic Medical Sciences, Hainan Academy of Medical Sciences Hainan Medical University  Haikou Hainan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2544-8293","authenticated-orcid":false,"given":"Xiao\u2010Yang","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Multi\u2010Organ Injury\u00a0Prevention and Treatment, Department of Developmental Biology, School of Basic Medical Sciences Southern Medical University  Guangzhou Guangdong China"},{"name":"School of Laboratory Medicine and Biotechnology Southern Medical University  Guangzhou Guangdong China"},{"name":"Key Laboratory of Mental Health of the Ministry of Education  Guangzhou Guangdong China"},{"name":"Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering Southern Medical University  Guangzhou Guangdong China"},{"name":"Guangdong\u2010Hong Kong Joint Laboratory for Psychiatric Disorders  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4390-2018","authenticated-orcid":false,"given":"Mei","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Multi\u2010Organ Injury\u00a0Prevention and Treatment, Department of Developmental Biology, School of Basic Medical Sciences Southern Medical University  Guangzhou Guangdong 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