{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T01:49:38Z","timestamp":1783561778886,"version":"3.55.0"},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,1,15]],"date-time":"2015-01-15T00:00:00Z","timestamp":1421280000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Respiratory J"],"published-print":{"date-parts":[[2016,9]]},"abstract":"Abstract<\/jats:title>Background and Aims<\/jats:title>Performing pleural biopsies during semi\u2010rigid thoracoscopy is sometimes a difficult and time\u2010consuming task because of the lack of mechanical power of dedicated flexible forceps in patients with thickened pleura. The purpose of this first ever pilot study was to test the feasibility of taking biopsy specimens by cryoprobe from the parietal pleura during semi\u2010rigid thoracoscopy. Our aim was also to assess the diagnostic value and quality of specimens obtained, morphological features, feasibility of immunohistochemistry staining and possibility of DNA isolation. The secondary aim was to evaluate safety, tolerability and duration of the procedure.<\/jats:p><\/jats:sec>Methods<\/jats:title>Fifteen patients with pleural effusion of unknown origin that underwent semi\u2010rigid thoracoscopy were included. Biopsies were obtained using a flexible autoclavable cryoprobe 20416\u2010032 (E<\/jats:styled-content>rbokryo CA<\/jats:styled-content>, ERBE<\/jats:styled-content>, T\u00fcbingen, G<\/jats:styled-content>ermany) 2.4\u2009mm in diameter and a semi\u2010rigid autoclavable O<\/jats:styled-content>lympus LTF<\/jats:styled-content>\u2010160 (O<\/jats:styled-content>lympus, Tokyo, J<\/jats:styled-content>apan) thoracoscope.<\/jats:p><\/jats:sec>Results<\/jats:title>Tissue samples were obtained from 14 patients (93.3%), three from each. Of the samples, 81% were easily interpretable and 19% were interpretable with some difficulty by the pathologist. The samples were of good quality, with the level of artifacts below 25%. The specimens were adequate for histological diagnosis, immunohistochemical staining and DNA<\/jats:styled-content> isolation. There were no moderate or major bleeding problems after the biopsies; two patients experienced pain. The median duration of three cryobiopsies (per patient) was 4\u2009min (range 3\u20136\u2009min).<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Cryobiopsy during semi\u2010rigid thoracoscopy appears worth to be evaluated in a larger prospective multicenter trial as our preliminary data were promising for efficacy and safety.<\/jats:p><\/jats:sec>","DOI":"10.1111\/crj.12256","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T03:47:33Z","timestamp":1418788053000},"page":"574-578","source":"Crossref","is-referenced-by-count":45,"title":["Feasibility and safety of parietal pleural cryobiopsy during semi\u2010rigid thoracoscopy"],"prefix":"10.1111","volume":"10","author":[{"given":"Ale\u0161","family":"Rozman","sequence":"first","affiliation":[{"name":"Endoscopy\/Pulmonology Department University Clinic of Pulmonary and Allergic Diseases Golnik Golnik Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luka","family":"Camlek","sequence":"additional","affiliation":[{"name":"Intensive Care Unit University Clinic of Pulmonary and Allergic Diseases Golnik Golnik Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mateja","family":"Marc Malovrh","sequence":"additional","affiliation":[{"name":"Department of Interventional Pulmonology University Clinic of Pulmonary and Allergic Diseases Golnik Golnik Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Izidor","family":"Kern","sequence":"additional","affiliation":[{"name":"Department of Pathology University Clinic of Pulmonary and Allergic Diseases Golnik Golnik Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Sch\u00f6nfeld","sequence":"additional","affiliation":[{"name":"Oberarzt Klinik f\u00fcr Pneumologie Lungenklinik Heckeshorn, HELIOS Klinikum Emil von Behring GmbH Berlin 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