{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:39:06Z","timestamp":1761489546121},"reference-count":18,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,3,19]],"date-time":"2015-03-19T00:00:00Z","timestamp":1426723200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"11th Five-Year National Major Special Project of Infectious Diseases","award":["2008ZX10301"],"award-info":[{"award-number":["2008ZX10301"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Respiratory J"],"published-print":{"date-parts":[[2016,9]]},"abstract":"Abstract<\/jats:title>Background and Aims<\/jats:title>A nanometer silicon membrane sandwich cup system was self\u2010designed. It could concentrate the bacilli via 0.45\u2010\u03bcm microporous filter membrane and semi\u2010automate the acid\u2010fast bacilli (AFB<\/jats:styled-content>) by a bacteria\u2010staining machine. The aim of the study was to assess the clinical value of our self\u2010designed system for diagnosing tuberculosis (TB<\/jats:styled-content>).<\/jats:p><\/jats:sec>Methods<\/jats:title>A total of 1993 sputum specimens obtained from patients with confirmed or suspected TB<\/jats:styled-content> were subjected to direct or concentrated specimens smear at X<\/jats:styled-content>iangY<\/jats:styled-content>a H<\/jats:styled-content>ospital, C<\/jats:styled-content>entral S<\/jats:styled-content>outh U<\/jats:styled-content>niversity between M<\/jats:styled-content>ay 2012 and F<\/jats:styled-content>ebruary 2013. In addition, all the specimens were also inoculated into L<\/jats:styled-content>owenstein\u2013J<\/jats:styled-content>ensen (L<\/jats:styled-content>\u2010J<\/jats:styled-content>) media, and culture results were considered as the gold standard for calculating sensitivity and specificity.<\/jats:p><\/jats:sec>Results<\/jats:title>Compared with direct smear examination, an increased density of red stained bacilli was observed in the self\u2010designed nanometer silicon membrane sandwich cup analysis under the microscope. The positive rate of the self\u2010designed analysis was significantly higher than that of direct AFB<\/jats:styled-content> smear [10.9% (217\/1993) vs 6.2% (123\/1993), P<\/jats:italic>\u2009<\u20090.05]. The sensitivity of the self\u2010designed system increased (97.3% vs 55.2%, P<\/jats:italic>\u2009<\u20090.05) without a loss of specificity (100% vs 100%) for identifying positive TB<\/jats:styled-content> cases compared with the direct smear method.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>The self\u2010designed nanometer silicon membrane sandwich cup and semi\u2010automatic bacteria\u2010staining machine could more efficiently and rapidly detect the AFB<\/jats:styled-content> in respiratory specimens than direct microscopy. This is a novel and safe examination, and it may replace direct smear examination for the diagnosis of patients with TB<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/crj.12273","type":"journal-article","created":{"date-parts":[[2015,1,26]],"date-time":"2015-01-26T09:18:41Z","timestamp":1422263921000},"page":"647-652","source":"Crossref","is-referenced-by-count":3,"title":["Evaluation of our self\u2010designed nanometer silicon membrane sandwich cup system for diagnosing tuberculosis"],"prefix":"10.1111","volume":"10","author":[{"given":"Jun","family":"Peng","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Xiangya Hospital, Central South University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen\u2010en","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Xiangya Hospital, Central South University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong\u2010Ling","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Xiangya Hospital, Central South University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing\u2010Xia","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Xiangya Hospital, Central South University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang\u2010Hui","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Xiangya Hospital, Central South University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ke","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Xiangya Hospital, Central South University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zi\u2010Juan","family":"Jian","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Xiangya Hospital, Central South University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan\u2010Bing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Xiangya Hospital, Central South University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wan\u2010Chan","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Xiangya Hospital, Central South University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,3,19]]},"reference":[{"issue":"4","key":"e_1_2_6_2_1","first-page":"3","article-title":"Tuberculosis: a global emergency","volume":"46","author":"Nakajima H","year":"1993","journal-title":"World Health"},{"key":"e_1_2_6_3_1","unstructured":"OrganizationWH.Global tuberculosis report 2013.2013."},{"key":"e_1_2_6_4_1","first-page":"3","article-title":"Report on fourth national epidemiological sampling survey of tuberculosis","volume":"25","author":"Duanmu H","year":"2002","journal-title":"Zhonghua Jie He He Hu Xi Za Zhi"},{"key":"e_1_2_6_5_1","unstructured":"Standards ATSCoRoD.Diagnostic standards and classification of tuberculosis in adults and children. 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