{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,15]],"date-time":"2026-02-15T08:01:48Z","timestamp":1771142508886,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,12,11]],"date-time":"2023-12-11T00:00:00Z","timestamp":1702252800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,12,11]],"date-time":"2023-12-11T00:00:00Z","timestamp":1702252800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Respiratory J"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The objective of this study is to study the adjunct role of combining DNA aneuploidy analysis with radial endobronchial ultrasound (R\u2010EBUS)\u2010guided sampling for diagnosis of peripheral lung lesions (PPLs).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>A single\u2010center prospective study was conducted in patients undergoing R\u2010EBUS\u2010guided sampling for PPLs. DNA image cytometry (DNA\u2010ICM) was used to analyze DNA aneuploidy in bronchial washing from the bronchial segment of the PPL. Clinical information, R\u2010EBUS data, pathology, DNA\u2010ICM results, and follow\u2010up data were analyzed. Sensitivity, specificity, and predictive values for R\u2010EBUS\u2010guided sampling, DNA\u2010ICM, and the two methods combined were measured. Binary logistic regression was performed to determine influencing factors on diagnostic positivity rate. Receiver operating characteristic (ROC) curve analysis was used to determine the optimal cutoff point for DNA\u2010ICM.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 101 patients were enrolled. Sixty\u2010four (63.4%) patients had confirmed malignant tumor, of whom 33 were confirmed by R\u2010EBUS\u2010guided sampling (biopsy and\/or bronchial brush and wash cytology), and 31 by surgery or percutaneous lung biopsy. Thirty\u2010seven patients were finally considered to have benign lesions, based on clinical information and 1\u2010year follow\u2010up. The sensitivity for malignant disease was 51.6% by R\u2010EBUS, and specificity was 100%. DNA\u2010ICM had a sensitivity of 67.2% and a specificity of 86.5%. When combining the two methods, sensitivity increased to 78.1% and specificity was 86.5%. Lesion size and whether the R\u2010EBUS probe was located in the lesion were significantly associated with positivity rate of the combined methods. The optimal cutoff point for DNA\u2010ICM was 5c for max DNA content, and 1 for aneuploid cell count (sensitivity 67.2%, specificity 86.5%, accuracy 63.4%).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>In malignant PPLs, DNA\u2010ICM combined with R\u2010EBUS\u2010guided sampling can improve diagnostic positivity compared with either method alone.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/crj.13703","type":"journal-article","created":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T15:26:34Z","timestamp":1702394794000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["DNA image cytometry of bronchial washing as a diagnostic adjunct to radial endobronchial ultrasound\u2010guided sampling of peripheral lung lesions: A single center prospective study"],"prefix":"10.1111","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6291-9915","authenticated-orcid":false,"given":"Cuiyan","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Peking University First Hospital  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Peking University First Hospital  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Peking University First Hospital  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junfang","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Peking University First Hospital  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