{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T15:23:03Z","timestamp":1769268183027,"version":"3.49.0"},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,5,23]],"date-time":"2024-05-23T00:00:00Z","timestamp":1716422400000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Respiratory J"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study aimed to explore the role and regulatory mechanism of lncRNA ZEB1\u2010AS1 in lung cancer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The expression of ZEB1\u2010AS1 and miR\u2010320b was determined by qRT\u2010PCR. Cell viability, proliferation migration, and invasion were assessed using the CCK\u20108, colony\u2010forming, and Transwell assay. EMT markers were quantified using western blot. The growth of subcutaneous tumor growth and metastatic bone tumors was evaluated in mouse model of lung cancer. Additionally, metastatic bone tumors were examined using H&amp;E staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>ZEB1\u2010AS1 expression was upregulated, while miR\u2010320b levels were downregulated in lung cancer. Knockdown of ZEB1\u2010AS1 resulted in a significant suppression of cell viability, proliferation, migration, invasion, and EMT in A549 cells. Furthermore, we confirmed the targeting relationship between ZEB1\u2010AS1 and miR\u2010320b, as well as between miR\u2010320b and BMPR1A. Our findings suggested that ZEB1\u2010AS1 regulated cell viability, proliferation, migration, and invasion, as well as EMT, in lung cancer cells by targeting the miR\u2010320b\/BMPR1A axis. Moreover, our in vivo experiments confirmed that ZEB1\u2010AS1 mediated bone metastasis through targeting miR\u2010320b\/BMPR1A axis in mice with lung cancer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>ZEB1\u2010AS1 mediated bone metastasis through targeting miR\u2010320b\/BMPR1A axis in lung cancer.<\/jats:p><\/jats:sec>","DOI":"10.1111\/crj.13770","type":"journal-article","created":{"date-parts":[[2024,5,24]],"date-time":"2024-05-24T06:00:01Z","timestamp":1716530401000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["ZEB1\u2010AS1 mediates bone metastasis through targeting miR\u2010320b\/BMPR1A axis in lung cancer"],"prefix":"10.1111","volume":"18","author":[{"given":"Nianxi","family":"Tan","sequence":"first","affiliation":[{"name":"Department of Cardiothoracic Vascular Surgery Zhuzhou Hospital Affiliated to Xiangya School of Medicine, Central South University  Zhuzhou Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junyi","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Vascular Surgery Zhuzhou Hospital Affiliated to Xiangya School of Medicine, Central South University  Zhuzhou Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Vascular Surgery Zhuzhou Hospital Affiliated to Xiangya School of Medicine, Central South University  Zhuzhou Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weilin","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Vascular Surgery Zhuzhou Hospital Affiliated to Xiangya School of Medicine, Central South University  Zhuzhou Hunan 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