{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T19:48:00Z","timestamp":1789501680279,"version":"build-2803163510"},"reference-count":31,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T00:00:00Z","timestamp":1715731200000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Respiratory J"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Hypertension is a main contributing factor of cardiovascular diseases; deregulated circular RNAs are involved in the pathogenesis of pulmonary arterial hypertension (PAH). Herein, we evaluated the function and mechanism of circST6GAL1 in PAH process.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Human pulmonary artery smooth muscle cells (HPASMCs) were cultured in hypoxic environment for functional analysis. The cell counting kit\u20108, 5\u2010ethynyl\u20102\u2032\u2010deoxyuridine, wound healing, and flow cytometry assays were used to investigate cell proliferation, migration, and apoptosis. qRT\u2010PCR and Western blotting analyses were used for level measurement of genes and proteins. The binding between miR\u2010509\u20105p and circST6GAL1 or multiple C2 and transmembrane domain containing 2 (MCTP2) was analyzed by dual\u2010luciferase reporter, RNA immunoprecipitation, and pull\u2010down assays. The monocrotaline (MCT)\u2010induced PAH mouse models were established for in vivo assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CircST6GAL1 was highly expressed in PAH patients and hypoxia\u2010induced HPASMCs. Functionally, circST6GAL1 deficiency reversed hypoxia\u2010induced proliferation and migration, as well as apoptosis arrest in HPASMCs. Mechanistically, circST6GAL1 directly targeted miR\u2010509\u20105p, and MCTP2 was a target of miR\u2010509\u20105p. Rescue assays showed that the regulatory effects of circST6GAL1 deficiency on hypoxia\u2010induced HPASMCs were abolished. Moreover, forced expression of miR\u2010509\u20105p suppressed HPASMC proliferation and migration and induced cell apoptosis under hypoxia stimulation, while these effects were abolished by MCTP2 overexpression. Moreover, circST6GAL1 silencing improved MCT\u2010induced pulmonary vascular remodeling and PAH.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>CircST6GAL1 deficiency reversed hypoxia\u2010induced proliferation and migration, as well as apoptosis arrest in HPASMCs, and alleviated pulmonary vascular remodeling in MCT\u2010induced PAH mouse models through the miR\u2010509\u20105p\/MCTP2 axis, indicating a potential therapeutic target for PAH.<\/jats:p><\/jats:sec>","DOI":"10.1111\/crj.13771","type":"journal-article","created":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T08:31:08Z","timestamp":1715761868000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["CircST6GAL1 knockdown alleviates pulmonary arterial hypertension by regulating miR\u2010509\u20105p\/multiple C2 and transmembrane domain containing 2 axis"],"prefix":"10.1111","volume":"18","author":[{"given":"Xing","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Peripheral Vascular Diseases The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Peripheral Vascular Diseases The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Peripheral Vascular Diseases The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junbo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Peripheral Vascular Diseases The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyan","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Peripheral Vascular Diseases The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-3789-4111","authenticated-orcid":false,"given":"Yan","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Peripheral Vascular Diseases The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,5,15]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20190835"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.117.029254"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/diseases6020038"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rmed.2020.106099"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045\u2010019\u20100776\u20108"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.27442"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010\u2010022\u201004543\u20109"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/15592294.2023.2210339"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0000000000002163"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12931\u2010023\u201002480\u20109"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12929\u2010023\u201000927\u20101"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/2045894019888416"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11993"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.07.014"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tjem.2019.09.002"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.202006\u20102169OC"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12931\u2010022\u201002248\u20107"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2019.03.012"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/5765029"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.111689"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41371\u2010023\u201000834\u2010w"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/21655979.2022.2036302"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.122.028299"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.32604\/or.2023.046582"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010021\u201000569\u20105"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.2478\/jtim\u20102023\u20100122"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20104939\u20106588\u20105_23"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12931\u2010018\u20100902\u20100"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11325\u2010022\u201002580\u20108"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/s41021\u2010022\u201000251\u20104"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384101.2022.2044147"}],"container-title":["The Clinical Respiratory Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/crj.13771","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T03:26:31Z","timestamp":1716953191000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/crj.13771"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5]]},"references-count":31,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2024,5]]}},"alternative-id":["10.1111\/crj.13771"],"URL":"https:\/\/doi.org\/10.1111\/crj.13771","archive":["Portico"],"relation":{},"ISSN":["1752-6981","1752-699X"],"issn-type":[{"value":"1752-6981","type":"print"},{"value":"1752-699X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5]]},"assertion":[{"value":"2024-01-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13771"}}