{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:29:38Z","timestamp":1753878578689,"version":"3.41.2"},"reference-count":42,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,8,20]],"date-time":"2024-08-20T00:00:00Z","timestamp":1724112000000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Respiratory J"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>COPD and bronchiectasis are common causes of morbidity, particularly around exacerbation. Colonisation with respiratory pathogens can increase the frequency and severity of exacerbations. However, bacterial and viral presence at exacerbation in people with airway colonisation has not been well studied.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A 6\u2010month cohort study of participants (<jats:italic>n<\/jats:italic>\u2009=\u200930) with chronic bronchitis due to bronchiectasis (<jats:italic>n<\/jats:italic>\u2009=\u200926) and\/or COPD (<jats:italic>n<\/jats:italic>\u2009=\u200913) and colonisation with <jats:styled-content style=\"fixed-case\"><jats:italic>Pseudomonas aeruginosa<\/jats:italic><\/jats:styled-content> or <jats:styled-content style=\"fixed-case\"><jats:italic>Haemophilus influenzae<\/jats:italic><\/jats:styled-content> was proven on two sputum cultures at exacerbation in the previous 12 months. Participants were provided self\u2010management education and collected sputum samples daily. Sputum samples at baseline (at least 14\u2009days before or after an exacerbation) and at each exacerbation were examined for a panel of 34 respiratory pathogens using commercially available RT\u2010PCR kits and compared to results obtained using culture methods for the detection of bacteria.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Participants provided 29 baseline samples and 71 samples at exacerbation. In 17\/29 baseline samples, RT\u2010PCR analysis confirmed the organism demonstrated by culture, while 12 samples showed a discrepancy from culture results. Most exacerbations (57.7%) were not associated with acquiring new bacteria or viruses, while 19.8% showed new bacteria, 15.7% new viruses and 7% both new viruses and bacteria.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Over half of exacerbations were not associated with new organisms in this cohort of participants with chronic bronchitis and colonisation. However, 26.8% demonstrated a new bacterial species in sputum, which is relevant for antibiotic therapy. Baseline RT\u2010PCR and culture results were discordant in one\u2010third of participants.<\/jats:p><\/jats:sec>","DOI":"10.1111\/crj.13811","type":"journal-article","created":{"date-parts":[[2024,8,20]],"date-time":"2024-08-20T10:38:06Z","timestamp":1724150286000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Respiratory Pathogens at Exacerbation in Chronic Bronchitis With Airway Bacterial Colonisation: A Cohort Study"],"prefix":"10.1111","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6324-7540","authenticated-orcid":false,"given":"Thomas\u00a0L.","family":"Jones","sequence":"first","affiliation":[{"name":"Department of Respiratory Medicine Portsmouth Hospitals University NHS Trust  Portsmouth UK"},{"name":"School of Pharmacy and Biomedical Sciences University of Portsmouth  Portsmouth UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claire","family":"Roberts","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Portsmouth Hospitals University NHS Trust  Portsmouth UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott","family":"Elliott","sequence":"additional","affiliation":[{"name":"Translational Research Laboratory Portsmouth Hospitals University NHS Trust  Portsmouth UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharon","family":"Glaysher","sequence":"additional","affiliation":[{"name":"Translational Research Laboratory Portsmouth Hospitals University NHS Trust  Portsmouth UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ben","family":"Green","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Portsmouth Hospitals University NHS Trust  Portsmouth UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janis\u00a0K.","family":"Shute","sequence":"additional","affiliation":[{"name":"School of Pharmacy and Biomedical Sciences University of Portsmouth  Portsmouth UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anoop\u00a0J.","family":"Chauhan","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Portsmouth Hospitals University NHS Trust  Portsmouth UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,8,20]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_10_2_1","DOI":"10.1183\/13993003.01033\u20102015"},{"doi-asserted-by":"publisher","key":"e_1_2_10_3_1","DOI":"10.1016\/J.CHEST.2018.07.014"},{"doi-asserted-by":"publisher","key":"e_1_2_10_4_1","DOI":"10.1183\/09059180.00003609"},{"doi-asserted-by":"publisher","key":"e_1_2_10_5_1","DOI":"10.1186\/S12890\u2010016\u20100238\u20109"},{"doi-asserted-by":"publisher","key":"e_1_2_10_6_1","DOI":"10.1183\/09059180.00002610"},{"doi-asserted-by":"publisher","key":"e_1_2_10_7_1","DOI":"10.1186\/1465\u20109921\u201013\u201021"},{"doi-asserted-by":"publisher","key":"e_1_2_10_8_1","DOI":"10.1136\/THORAX.57.9.759"},{"doi-asserted-by":"publisher","key":"e_1_2_10_9_1","DOI":"10.1186\/1471\u20102466\u201014\u2010103"},{"doi-asserted-by":"publisher","key":"e_1_2_10_10_1","DOI":"10.1016\/S0928\u20108244(03)00100\u20107"},{"doi-asserted-by":"publisher","key":"e_1_2_10_11_1","DOI":"10.1513\/AnnalsATS.201310\u2010335OC"},{"doi-asserted-by":"publisher","key":"e_1_2_10_12_1","DOI":"10.1128\/CMR.14.2.336\u2010363.2001"},{"doi-asserted-by":"publisher","key":"e_1_2_10_13_1","DOI":"10.1183\/16000617.0048\u20102019"},{"unstructured":"A. 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