{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T13:49:09Z","timestamp":1776692949315,"version":"3.51.2"},"reference-count":29,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T00:00:00Z","timestamp":1764374400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T00:00:00Z","timestamp":1764374400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Respiratory J"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Vascular alterations contribute significantly to the chronic obstructive pulmonary disease (COPD) pathophysiology. Thirty\u2010nine percent of patients with COPD develop pulmonary hypertension, especially patients with the severe form of the disease. Cigarette\u2010induced endothelial dysfunction is important in the pathogenesis of vascular alterations, even in the mild forms of the disease. This study investigates extracellular matrix (ECM) remodelling and endothelial activation in pulmonary (PMA) and bronchial muscular arteries (BMA) from non\u2010smokers (NS), nonobstructed smokers (NOS) and patients with mild\/moderate COPD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Lung tissue samples from 44 patients undergoing lung resection were analysed. Morphometric parameters, ECM components (collagens, fibronectin, elastic fibres, tenascin\u2010C and versican) and endothelial markers (VCAM\u20101, ICAM\u20101 and endothelin\u20101) were quantified in BMA and PMA using immunohistochemistry and morphometric analysis. Group differences and correlations with clinical parameters were assessed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      COPD patients showed increased intimal thickness and fibronectin deposition in PMA, and larger adventitial areas in BMA compared to NS. NOS exhibited higher VCAM\u20101 expression in BMA and increased elastic fibre content in PMA. In COPD, elastic fibres and type\u2010III collagen negatively correlated with smoking history (pack\u2010years), while fibronectin positively correlated with age. VCAM\u20101 expression in BMA correlated negatively with lung function (FEV\n                      <jats:sub>1<\/jats:sub>\n                      and FEV\n                      <jats:sub>1<\/jats:sub>\n                      \/FVC).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study demonstrates for the first time ECM remodelling and endothelial activation in bronchial arteries of smokers and patients with COPD. Fibronectin emerges as a key ECM component in arterial remodelling in mild\u2013moderate COPD. Understanding vascular changes may provide new insights into the regulation of bronchial circulation and the development of pulmonary hypertension in COPD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/crj.70141","type":"journal-article","created":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T11:16:14Z","timestamp":1764414974000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Extracellular Matrix and Endothelial Activation Markers in the Bronchial and Pulmonary Arteries in COPD"],"prefix":"10.1111","volume":"19","author":[{"given":"Raquel","family":"Annoni","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Patologia Ambiental e Experimental, Departamento de Patologia Faculdade de Medicina da Universidade de S\u00e3o Paulo  S\u00e3o Paulo SP Brazil"},{"name":"Graduate Program in Rehabilitation Sciences, School of Physical Education, Physical Therapy and Occupational Therapy Universidade Federal de Minas Gerais  Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7934-7191","authenticated-orcid":false,"given":"J\u00f4se\u00a0M\u00e1ra","family":"de Brito","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia Ambiental e Experimental, Departamento de Patologia Faculdade de Medicina da Universidade de S\u00e3o Paulo  S\u00e3o Paulo SP Brazil"},{"name":"Instituto B\u00e1sico de Bioci\u00eancias, Universidade de Taubat\u00e9 (UNITAU)  Taubat\u00e9 SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salvatore","family":"Battaglia","sequence":"additional","affiliation":[{"name":"Division of Respiratory Diseases Department of Promozione della Salute, Materno Infantile Medicina Interna e Specialistica di Eccellenza 'G. D'Alessandro' \u2013 PROMISE \u2013 University of Palermo  Palermo Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nat\u00e1lia","family":"de Souza\u00a0Xavier\u00a0Costa","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia Ambiental e Experimental, Departamento de Patologia Faculdade de Medicina da Universidade de S\u00e3o Paulo  S\u00e3o Paulo SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ligia\u00a0Braga\u00a0Lopes","family":"Couceiro","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia Ambiental e Experimental, Departamento de Patologia Faculdade de Medicina da Universidade de S\u00e3o Paulo  S\u00e3o Paulo SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renata\u00a0Calciolari","family":"Rossi","sequence":"additional","affiliation":[{"name":"Departamento de Patologia Faculdade de Medicina da Universidade do Oeste Paulista  Presidente Prudente SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marisa","family":"Dolhnikoff","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia Ambiental e Experimental, Departamento de Patologia Faculdade de Medicina da Universidade de S\u00e3o Paulo  S\u00e3o Paulo SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pieter\u00a0S.","family":"Hiemstra","sequence":"additional","affiliation":[{"name":"Department of Pulmonology Leiden University Medical Center  Leiden The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7020-1401","authenticated-orcid":false,"given":"Klaus\u00a0F.","family":"Rabe","sequence":"additional","affiliation":[{"name":"LungenClinic Grosshansdorf, Member of the German Center for Lung Research (DZL)  Grosshansdorf Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter\u00a0J.","family":"Sterk","sequence":"additional","affiliation":[{"name":"Professor Emeritus University of Amsterdam  Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3354-1466","authenticated-orcid":false,"given":"Thais","family":"Mauad","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia Ambiental e Experimental, Departamento de Patologia Faculdade de Medicina da Universidade de S\u00e3o Paulo  S\u00e3o Paulo SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,29]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells8040342"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00332.2023"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/resp.12772"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.2147\/COPD.S359873"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.02.00245902"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxics10060312"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1465\u20109921\u201014\u201047"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2012.02.029"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1148\/rg.351140089"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/thx.2008.100032"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002\u20109343(69)90070\u20109"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/1465\u20109921\u201011\u2010132"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41569\u2010024\u201001103\u20100"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00192611"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.160.3.9809088"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00147807"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00408\u2010015\u20109717\u20107"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201401\u20100037OC"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.102.6.1645"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1177\/000331970405500406"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010\u2010014\u20102231\u2010z"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13223\u2010019\u20100363\u20100"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.57.030195.000525"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1177\/2045894018758528"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.21037\/atm\u201022\u20102523"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1183\/23120541.00354\u20102023"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2020.09.005"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00022.2024"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00015608"}],"container-title":["The Clinical Respiratory Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/crj.70141","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/crj.70141","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/crj.70141","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T09:21:13Z","timestamp":1768209673000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/crj.70141"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,29]]},"references-count":29,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1111\/crj.70141"],"URL":"https:\/\/doi.org\/10.1111\/crj.70141","archive":["Portico"],"relation":{},"ISSN":["1752-6981","1752-699X"],"issn-type":[{"value":"1752-6981","type":"print"},{"value":"1752-699X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,29]]},"assertion":[{"value":"2025-10-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70141"}}