{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T12:19:29Z","timestamp":1768220369631,"version":"3.49.0"},"reference-count":18,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,7]],"date-time":"2025-12-07T00:00:00Z","timestamp":1765065600000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["1\u2010P306\u2010M\u2010206932\u201001A1"],"award-info":[{"award-number":["1\u2010P306\u2010M\u2010206932\u201001A1"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Respiratory J"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>There are no treatments directly targeting the pulmonary vasculature in chronic obstructive pulmonary disease (COPD), and further characterization of the underlying endothelial cell (EC) abnormalities could be helpful in drug development.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We investigated the influence of exercise and the prostacyclin analog iloprost on extracellular vesicles derived from ECs (eEVs) in 15 moderate\u2013severe COPD patients who were enrolled in a randomized, placebo\u2010controlled crossover trial of iloprost.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Active smokers had a profile consistent with inflammatory\u2010derived EVs, while exacerbation\u2010prone COPD subjects had a profile consistent with apoptosis\u2010derived eEVs. There were no significant effects of iloprost on eEV levels. However, there was a significant increase in CD144+ and CD31+\/CD144+ EVs 1\u2009h after exercise.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Endothelial\u2010derived EV profiles differed based on smoking and exacerbation history. Iloprost did not affect eEV levels, although maximal exercise induced a delayed increase in a subset of eEVs, possibly through shear stress.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/crj.70146","type":"journal-article","created":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T03:04:56Z","timestamp":1765163096000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Endothelial\u2010Derived Extracellular Vesicles During Exercise in COPD Patients"],"prefix":"10.1111","volume":"19","author":[{"given":"Samuel\u00a0C.","family":"Okpechi","sequence":"first","affiliation":[{"name":"Stanley S. Scott Cancer Center Louisiana State University Health Sciences Center  New Orleans Louisiana USA"},{"name":"Department of Biochemistry and Molecular Biology Louisiana State University Health Sciences Center and School of Medicine  New Orleans Louisiana USA"},{"name":"Cancer Research Technology Program, Molecular Pharmacology Program Frederick National Laboratory for Cancer Research  Frederick Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorota","family":"Wyczechowska","sequence":"additional","affiliation":[{"name":"Stanley S. Scott Cancer Center Louisiana State University Health Sciences Center  New Orleans Louisiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bennett","family":"DeBoisblanc","sequence":"additional","affiliation":[{"name":"Section of Pulmonary\/Critical Care and Allergy\/Immunology Louisiana State University Health Sciences Center  New Orleans Louisiana USA"},{"name":"Comprehensive Pulmonary Hypertension Center University Medical Center  New Orleans Louisiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica\u00a0L.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Comprehensive Pulmonary Hypertension Center University Medical Center  New Orleans Louisiana USA"},{"name":"College of Health and Pharmacy Husson University School of Pharmacy  Bangor Maine USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed\u00a0A.","family":"Ghonim","sequence":"additional","affiliation":[{"name":"Stanley S. Scott Cancer Center Louisiana State University Health Sciences Center  New Orleans Louisiana USA"},{"name":"The Department of Microbiology and Immunology, Faculty of Pharmacy Al\u2010Azhar University  Cairo Egypt"},{"name":"Department of Host\u2010Microbe Interactions St. Jude Children's Research Hospital  Memphis Tennessee USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalie","family":"Bauer","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Center for Lung Biology University of South Alabama College of Medicine  Mobile Alabama USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hamid\u00a0A.","family":"Boulares","sequence":"additional","affiliation":[{"name":"Stanley S. Scott Cancer Center Louisiana State University Health Sciences Center  New Orleans Louisiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew\u00a0R.","family":"Lammi","sequence":"additional","affiliation":[{"name":"Section of Pulmonary\/Critical Care and Allergy\/Immunology Louisiana State University Health Sciences Center  New Orleans Louisiana USA"},{"name":"Comprehensive Pulmonary Hypertension Center University Medical Center  New Orleans Louisiana USA"},{"name":"Division of Pulmonary and Critical Care Medicine Johns Hopkins University School of Medicine  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,7]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl\u20102011\u2010201395"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201209\u20101697OC"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21072514"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12931\u2010024\u201002719\u2010z"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm10215014"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.02.00094302"},{"issue":"9","key":"e_1_2_11_8_1","first-page":"197","article-title":"The Influence of Iloprost on Blood Rheology and Tissue Perfusion in Patients With Intermittent Claudication","volume":"59","author":"Ciuffetti G.","year":"2003","journal-title":"Kardiologia Polska"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rmed.2021.106354"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/resp.13500"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v3.26913"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0049\u20103848(03)00064\u20101"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1113\/ep086644"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00516.2016"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010017\u201017249\u20106"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00047.2009"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jex2.59"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010022\u201005161\u20107"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.36416\/1806\u20103756\/e20200007"}],"container-title":["The Clinical Respiratory Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/crj.70146","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/crj.70146","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/crj.70146","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T09:21:23Z","timestamp":1768209683000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/crj.70146"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":18,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1111\/crj.70146"],"URL":"https:\/\/doi.org\/10.1111\/crj.70146","archive":["Portico"],"relation":{},"ISSN":["1752-6981","1752-699X"],"issn-type":[{"value":"1752-6981","type":"print"},{"value":"1752-699X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"2025-09-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70146"}}