{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T11:21:11Z","timestamp":1774437671400,"version":"3.50.1"},"reference-count":19,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,12,26]],"date-time":"2013-12-26T00:00:00Z","timestamp":1388016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Transplantation"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Previously, we have reported that flow cytometry analysis of fine\u2010needle aspirates can accurately predict rejection in kidney transplants treated with cyclosporine\u2013azathioprine\u2013prednisolone. In this study, we examined this technique's accuracy using current immunosuppression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Kidney transplant recipients were treated with calcineurin inhibitors, mycophenolate mofetil, and prednisolone: 92 remained rejection\u2010free \u2013 Group I \u2013 and 37 developed acute rejection \u2013 Group <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>. An allograft aspiration specimen and peripheral blood were collected from Group I on post\u2010transplant day 7 and from Group <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> on the day of clinical rejection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Significant changes were seen in both aspiration and peripheral blood samples in several T cell subsets when comparing Groups I and <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>. A sensitivity of 94.6%, specificity of 85%, and <jats:styled-content style=\"fixed-case\">AUC<\/jats:styled-content>\u00a0=\u00a00.966 were observed through combining <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:styled-content style=\"fixed-case\">DR<\/jats:styled-content> with <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>3<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>69 values from aspiration specimen; the corresponding <jats:styled-content style=\"fixed-case\">AUC<\/jats:styled-content> in peripheral blood was 0.847. Irreversible rejections displayed a significantly higher activation score (p\u00a0=\u00a00.024).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Flow cytometry analysis of aspiration specimen achieved high diagnostic performance in renal transplants through studying <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:styled-content style=\"fixed-case\">DR<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>3<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>69 under current immunosuppressive therapy. Peripheral blood analysis, although not significant, showed the same trend. The activation score anticipated the irreversibility of rejection. The data suggest this test, through an easily tolerated technique, merits further diagnostic use.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ctr.12293","type":"journal-article","created":{"date-parts":[[2013,12,26]],"date-time":"2013-12-26T12:19:34Z","timestamp":1388060374000},"page":"177-183","source":"Crossref","is-referenced-by-count":6,"title":["Flow cytometry assessment of graft\u2010infiltrating lymphocytes can accurately identify acute rejection in kidney transplants"],"prefix":"10.1111","volume":"28","author":[{"given":"Paula D.P.","family":"Xavier","sequence":"first","affiliation":[{"name":"Centro de Histocompatibilidade do Norte Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gloria L.","family":"Lema","sequence":"additional","affiliation":[{"name":"Unidad de Trasplantes Hospital Pablo Tob\u00f3n Uribe Medell\u00edn Colombia"},{"name":"Epidemiology Academic Group (GRAEPIC) Universidad de Antioquia Medell\u00edn Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria C.","family":"Magalh\u00e3es","sequence":"additional","affiliation":[{"name":"Servi\u00e7o Imunologia Hospital S. Jo\u00e3o Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Armando","family":"Teixeira\u2010Pinto","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Bioestat\u00edstica e Inform\u00e1tica M\u00e9dica CINTESIS Faculdade De Medicina da Universidade Porto Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susana","family":"Sampaio\u2010Norton","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Nefrologia Hospital S. Jo\u00e3o Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00e9rgio","family":"Gai\u00e3o","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Nefrologia Hospital S. Jo\u00e3o Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiago","family":"Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Servi\u00e7o Imunologia Hospital S. 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