{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T08:10:31Z","timestamp":1793261431668,"version":"4.5.0"},"reference-count":49,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2014,7,22]],"date-time":"2014-07-22T00:00:00Z","timestamp":1405987200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SFB 738"],"award-info":[{"award-number":["SFB 738"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Transplantation"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Acute rejection (AR) remains a major cause for long\u2010term kidney allograft failure. Reliable immunological parameters suitable to define the pre\u2010transplant immune state and hence the individual risk of graft rejection are highly desired to preferably adapt the immunosuppressive regimen in advance. Donor and third party alloreactivities were determined by mixed lymphocyte cultures. Soluble forms of CD25, CD30, and CD44 were detected in patients' serum by ELISA. Various lymphocyte subpopulations were measured using flow cytometry. All patients received triple immunosuppression (tacrolimus\/mycophenolate mofetil\/steroids) and were grouped according to biopsy results within the first year: rejection\u2010free (RF, n\u00a0=\u00a013), borderline (BL, n\u00a0=\u00a05), or acute rejection (AR, n\u00a0=\u00a07). Patients with AR showed the highest pre\u2010transplant alloreactivities and serum levels (<jats:styled-content style=\"fixed-case\">sCD<\/jats:styled-content>25\/<jats:styled-content style=\"fixed-case\">sCD<\/jats:styled-content>30\/<jats:styled-content style=\"fixed-case\">sCD<\/jats:styled-content>44) according to the pattern RF\u00a0&lt;\u00a0BL\u00a0&lt;\u00a0AR. Relying on serum analysis only, multivariate logistic regression (logit link function) yielded a prognostic score for prediction of rejection with 75.0% sensitivity and 69.2% specificity. Patients with rejection showed markedly higher pre\u2010transplant frequencies of CD4<jats:sup>+<\/jats:sup>\/CD8<jats:sup>+<\/jats:sup> T cells lacking CD28, but lower numbers of CD8<jats:sup>+<\/jats:sup>CD161<jats:sup>bright<\/jats:sup> T cells and NK cells than RF individuals. Pre\u2010transplant immune state defined by alloreactivity, serum markers, and particular lymphocyte subsets seems to correlate with occurrence of graft rejection after kidney transplantation. A prognostic score based on pre\u2010transplant serum levels has shown great potential for prediction of rejection episodes and should be further evaluated.<\/jats:p>","DOI":"10.1111\/ctr.12399","type":"journal-article","created":{"date-parts":[[2014,6,13]],"date-time":"2014-06-13T11:53:54Z","timestamp":1402660434000},"page":"968-979","source":"Crossref","is-referenced-by-count":13,"title":["Pre\u2010transplant immune state defined by serum markers and alloreactivity predicts acute rejection after living donor kidney transplantation"],"prefix":"10.1111","volume":"28","author":[{"given":"Florian W.R.","family":"Vondran","sequence":"first","affiliation":[{"name":"Transplant Laboratory Department of General, Visceral and Transplant Surgery Hannover Medical School Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Timrott","sequence":"additional","affiliation":[{"name":"Transplant Laboratory Department of General, Visceral and Transplant Surgery Hannover Medical School Hannover Germany"},{"name":"Integrated Research and Treatment Center Transplantation (IFB\u2010Tx) Hannover Medical School Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonja","family":"Kollrich","sequence":"additional","affiliation":[{"name":"Transplant Laboratory Department of General, Visceral and Transplant Surgery Hannover Medical School Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann\u2010Kristin","family":"Steinhoff","sequence":"additional","affiliation":[{"name":"Transplant Laboratory Department of General, Visceral and Transplant Surgery Hannover Medical School Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Kaltenborn","sequence":"additional","affiliation":[{"name":"Transplant Laboratory Department of General, Visceral and Transplant Surgery Hannover Medical School Hannover Germany"},{"name":"Integrated Research and Treatment Center Transplantation (IFB\u2010Tx) Hannover Medical School Hannover Germany"},{"name":"Department of Orthopedic and Trauma Surgery Federal Armed Forces Hospital Westerstede Westerstede Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harald","family":"Schrem","sequence":"additional","affiliation":[{"name":"Transplant Laboratory Department of General, Visceral and Transplant Surgery Hannover Medical School Hannover Germany"},{"name":"Integrated Research and Treatment Center Transplantation (IFB\u2010Tx) Hannover Medical School Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juergen","family":"Klempnauer","sequence":"additional","affiliation":[{"name":"Transplant Laboratory Department of General, Visceral and Transplant Surgery Hannover Medical School Hannover Germany"},{"name":"Integrated Research and Treatment Center Transplantation (IFB\u2010Tx) Hannover Medical School Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Lehner","sequence":"additional","affiliation":[{"name":"Transplant Laboratory Department of General, Visceral and Transplant Surgery Hannover Medical School Hannover Germany"},{"name":"Integrated Research and Treatment Center Transplantation (IFB\u2010Tx) Hannover Medical School Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reinhard","family":"Schwinzer","sequence":"additional","affiliation":[{"name":"Transplant Laboratory Department of General, Visceral and Transplant Surgery Hannover Medical School Hannover Germany"},{"name":"Integrated Research and Treatment Center Transplantation (IFB\u2010Tx) Hannover Medical School Hannover 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