{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T09:07:30Z","timestamp":1791191250860,"version":"4.1.0"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,3,13]],"date-time":"2015-03-13T00:00:00Z","timestamp":1426204800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Transplantation"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Class I single\u2010antigen flow beads (<jats:styled-content style=\"fixed-case\">SAFB<\/jats:styled-content>) carry native and denatured human leukocyte antigen (<jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>) molecules. Using a cohort of 179 class I <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>\u2010sensitized kidney recipients, we described incidence and clinical relevance of preformed denatured <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content> donor\u2010specific antibodies (<jats:styled-content style=\"fixed-case\">DSA<\/jats:styled-content>) using two different assays: an acid\u2010treated <jats:styled-content style=\"fixed-case\">SAFB<\/jats:styled-content> assay (anti\u2010<jats:styled-content style=\"fixed-case\">dHLA DSA<\/jats:styled-content>) and the iBeads assays (<jats:styled-content style=\"fixed-case\">SAFB<\/jats:styled-content>+\/iBeads\u2010 <jats:styled-content style=\"fixed-case\">DSA<\/jats:styled-content>). Eighty\u2010five class I <jats:styled-content style=\"fixed-case\">DSA<\/jats:styled-content> were found in 67 patients (median mean fluorescence intensity [<jats:styled-content style=\"fixed-case\">MFI<\/jats:styled-content>] of 1729 [range 520\u201313\u00a0882]). Anti\u2010dHLA and <jats:styled-content style=\"fixed-case\">SAFB<\/jats:styled-content>+\/iBeads\u2010 <jats:styled-content style=\"fixed-case\">DSA<\/jats:styled-content> represented 11% and 18% of class I <jats:styled-content style=\"fixed-case\">DSA<\/jats:styled-content> and were mainly low <jats:styled-content style=\"fixed-case\">MFI DSA<\/jats:styled-content> (500\u20131000 <jats:styled-content style=\"fixed-case\">MFI<\/jats:styled-content>). Concordance between these two assays was good (90%). None of the patients with only class I anti\u2010<jats:styled-content style=\"fixed-case\">dHLA DSA<\/jats:styled-content> or only <jats:styled-content style=\"fixed-case\">SAFB<\/jats:styled-content>+\/iBeads\u2010 <jats:styled-content style=\"fixed-case\">DSA<\/jats:styled-content> developed acute clinical antibody\u2010mediated rejection in the first\u2010year post\u2010transplantation, and their five\u2010yr death\u2010censored graft survival was similar to that of patients without <jats:styled-content style=\"fixed-case\">DSA<\/jats:styled-content>. Moreover, all these patients displayed a negative current T\u2010cell flow cytometry cross\u2010match. Therefore, both anti\u2010dHLA <jats:styled-content style=\"fixed-case\">DSA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">SAFB<\/jats:styled-content>+\/iBeads\u2010 <jats:styled-content style=\"fixed-case\">DSA<\/jats:styled-content> appear irrelevant, which could explain the good outcome observed in some patients with preformed class I DSA.<\/jats:p>","DOI":"10.1111\/ctr.12529","type":"journal-article","created":{"date-parts":[[2015,2,13]],"date-time":"2015-02-13T12:37:13Z","timestamp":1423831033000},"page":"393-402","source":"Crossref","is-referenced-by-count":41,"title":["Clinical impact of preformed donor\u2010specific denatured class I <scp>HLA<\/scp> antibodies after kidney transplantation"],"prefix":"10.1111","volume":"29","author":[{"given":"Jonathan","family":"Visentin","sequence":"first","affiliation":[{"name":"Laboratoire d'Immunologie et Immunog\u00e9n\u00e9tique H\u00f4pital Pellegrin CHU de Bordeaux Bordeaux France"},{"name":"UMR CNRS 5164 Universit\u00e9 de Bordeaux Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e9lanie","family":"Marroc","sequence":"additional","affiliation":[{"name":"Service de N\u00e9phrologie, Transplantation, Dialyse H\u00f4pital Pellegrin CHU de Bordeaux Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gwendaline","family":"Guidicelli","sequence":"additional","affiliation":[{"name":"Laboratoire d'Immunologie et Immunog\u00e9n\u00e9tique H\u00f4pital Pellegrin CHU de Bordeaux Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Bachelet","sequence":"additional","affiliation":[{"name":"UMR CNRS 5164 Universit\u00e9 de Bordeaux Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thoa","family":"Nong","sequence":"additional","affiliation":[{"name":"One Lambda, Inc. Canoga Park CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Fran\u00e7ois","family":"Moreau","sequence":"additional","affiliation":[{"name":"Laboratoire d'Immunologie et Immunog\u00e9n\u00e9tique H\u00f4pital Pellegrin CHU de Bordeaux Bordeaux France"},{"name":"UMR CNRS 5164 Universit\u00e9 de Bordeaux Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jar\u2010How","family":"Lee","sequence":"additional","affiliation":[{"name":"One Lambda, Inc. Canoga Park CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Merville","sequence":"additional","affiliation":[{"name":"UMR CNRS 5164 Universit\u00e9 de Bordeaux Bordeaux France"},{"name":"Service de N\u00e9phrologie, Transplantation, Dialyse H\u00f4pital Pellegrin CHU de Bordeaux Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lionel","family":"Couzi","sequence":"additional","affiliation":[{"name":"UMR CNRS 5164 Universit\u00e9 de Bordeaux Bordeaux France"},{"name":"Service de N\u00e9phrologie, Transplantation, Dialyse H\u00f4pital Pellegrin CHU de Bordeaux Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Luc","family":"Taupin","sequence":"additional","affiliation":[{"name":"Laboratoire d'Immunologie et Immunog\u00e9n\u00e9tique H\u00f4pital Pellegrin CHU de Bordeaux Bordeaux France"},{"name":"UMR CNRS 5164 Universit\u00e9 de Bordeaux Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,3,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2011.03640.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2009101065"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2009.02985.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2012070664"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2012.04291.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2009.06.020"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/cei.12127"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000000229"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(93)90037-C"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.4.1918"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-3274(96)80011-6"},{"key":"e_1_2_7_13_1","doi-asserted-by":"crossref","first-page":"3533","DOI":"10.4049\/jimmunol.153.8.3533","article-title":"Beta 2\u2010microglobulin induces a conformational change in an MHC class I H chain that occurs intracellularly and is maintained at the cell surface","volume":"153","author":"Danliczyk UG","year":"1994","journal-title":"J Immunol"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2011.02.012"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e3181ac6198"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2009.02987.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.12590"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e31820794bb"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-6143.3.s4.11.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2011.03584.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-2277.2011.01408.x"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trim.2010.12.003"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2012.07.320"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.12709"}],"container-title":["Clinical Transplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fctr.12529","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ctr.12529","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T15:28:44Z","timestamp":1696346924000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ctr.12529"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,3,13]]},"references-count":23,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2015,5]]}},"alternative-id":["10.1111\/ctr.12529"],"URL":"https:\/\/doi.org\/10.1111\/ctr.12529","archive":["Portico"],"relation":{},"ISSN":["0902-0063","1399-0012"],"issn-type":[{"value":"0902-0063","type":"print"},{"value":"1399-0012","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,3,13]]}}}