{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T12:31:41Z","timestamp":1763037101408,"version":"3.41.2"},"reference-count":17,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2017,8,30]],"date-time":"2017-08-30T00:00:00Z","timestamp":1504051200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000102","name":"Health Resources and Services Administration","doi-asserted-by":"publisher","award":["234\u20102005\u201037011C"],"award-info":[{"award-number":["234\u20102005\u201037011C"]}],"id":[{"id":"10.13039\/100000102","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Transplantation"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Complement\u2010dependent cytotoxicity cross\u2010match (<jats:styled-content style=\"fixed-case\">CDCXM<\/jats:styled-content>) is used for evaluation of preformed <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>\u2010specific antibodies in patients undergoing heart transplantation. Flow cytometry cross\u2010match (<jats:styled-content style=\"fixed-case\">FCXM<\/jats:styled-content>) is a more sensitive assay and used with increasing frequency. To determine the clinical relevance of a positive <jats:styled-content style=\"fixed-case\">FCXM<\/jats:styled-content> in the context of negative <jats:styled-content style=\"fixed-case\">CDCXM<\/jats:styled-content> in heart transplantation, the United Network for Organ Sharing (<jats:styled-content style=\"fixed-case\">UNOS<\/jats:styled-content>) database was analyzed. Kaplan\u2010Meier analysis and Cox proportional hazard modeling were used to assess graft survival for three different patient cohorts defined by cross\u2010match results: T\u2010cell and B\u2010cell <jats:styled-content style=\"fixed-case\">CDCXM<\/jats:styled-content>+ (\u201c<jats:styled-content style=\"fixed-case\">CDCXM<\/jats:styled-content>+\u201d cohort), <jats:styled-content style=\"fixed-case\">CDCXM<\/jats:styled-content>\u2212 but T\u2010cell and\/or B\u2010cell <jats:styled-content style=\"fixed-case\">FCXM<\/jats:styled-content>+ (\u201c<jats:styled-content style=\"fixed-case\">FCXM<\/jats:styled-content>+\u201d cohort), and T\u2010cell\/B\u2010cell <jats:styled-content style=\"fixed-case\">CDCXM<\/jats:styled-content>\u2212 and <jats:styled-content style=\"fixed-case\">FCXM<\/jats:styled-content>\u2010 (\u201c<jats:styled-content style=\"fixed-case\">XM<\/jats:styled-content>\u2212\u201d cohort). During the study period, 2558 patients met inclusion criteria (10.7% <jats:styled-content style=\"fixed-case\">CDCXM<\/jats:styled-content>+, 18.8% <jats:styled-content style=\"fixed-case\">FCXM<\/jats:styled-content>+, 65.5% <jats:styled-content style=\"fixed-case\">XM<\/jats:styled-content>\u2212). <jats:styled-content style=\"fixed-case\">CDCXM<\/jats:styled-content>+ patients had significantly decreased graft survival compared to <jats:styled-content style=\"fixed-case\">FCXM<\/jats:styled-content>+ and <jats:styled-content style=\"fixed-case\">XM<\/jats:styled-content>\u2212 cohorts (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.003 and &lt;.001, respectively). <jats:styled-content style=\"fixed-case\">CDCXM<\/jats:styled-content>\u2212 and <jats:styled-content style=\"fixed-case\">FCXM<\/jats:styled-content>+ patients did not have decreased graft survival compared to <jats:styled-content style=\"fixed-case\">XM<\/jats:styled-content>\u2212 patients (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.09). In multivariate analysis, only <jats:styled-content style=\"fixed-case\">CDCXM<\/jats:styled-content>+ was associated with decreased graft survival (<jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content> 1.22, 95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content> 1.01\u20101.49). In conclusion, positive <jats:styled-content style=\"fixed-case\">FCXM<\/jats:styled-content> in the context of negative <jats:styled-content style=\"fixed-case\">CDCXM<\/jats:styled-content> does not confer increased risk of graft failure. Further study is needed to understand implications of <jats:styled-content style=\"fixed-case\">CDCXM<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">FCXM<\/jats:styled-content> testing in heart transplant recipients.<\/jats:p>","DOI":"10.1111\/ctr.13064","type":"journal-article","created":{"date-parts":[[2017,8,2]],"date-time":"2017-08-02T09:39:39Z","timestamp":1501666779000},"source":"Crossref","is-referenced-by-count":5,"title":["Value of a flow cytometry cross\u2010match in the setting of a negative complement\u2010dependent cytotoxicity cross\u2010match in heart transplant recipients"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4065-1698","authenticated-orcid":false,"given":"Britton C.","family":"Keeshan","sequence":"first","affiliation":[{"name":"Division of Pediatric Cardiology Seattle Children's Hospital  Seattle WA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew J.","family":"O'Connor","sequence":"additional","affiliation":[{"name":"Division of Cardiology The Children's Hospital of Philadelphia  Philadelphia PA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimberly Y.","family":"Lin","sequence":"additional","affiliation":[{"name":"Division of Cardiology The Children's Hospital of Philadelphia  Philadelphia PA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dimitrios","family":"Monos","sequence":"additional","affiliation":[{"name":"Immunogenetics Laboratory The Children's Hospital of Philadelphia  Philadelphia PA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Curt","family":"Lind","sequence":"additional","affiliation":[{"name":"Immunogenetics Laboratory The Children's Hospital of Philadelphia  Philadelphia PA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher E.","family":"Mascio","sequence":"additional","affiliation":[{"name":"Division of Cardiothoracic Surgery The Children's Hospital of Philadelphia  Philadelphia PA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jesus Eduardo","family":"Rame","sequence":"additional","affiliation":[{"name":"Division of Cardiology Department of Medicine University of Pennsylvania  Philadelphia PA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas L.","family":"Spray","sequence":"additional","affiliation":[{"name":"Division of Cardiothoracic Surgery The Children's Hospital of Philadelphia  Philadelphia PA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert E.","family":"Shaddy","sequence":"additional","affiliation":[{"name":"Division of Cardiology The Children's Hospital of Philadelphia  Philadelphia PA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph W.","family":"Rossano","sequence":"additional","affiliation":[{"name":"Division of Cardiology The Children's Hospital of Philadelphia  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USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,8,30]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2009.04.016"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trim.2013.05.001"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trim.2004.01.005"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2009.06.009"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2009.06.015"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-200010150-00011"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2011.08.077"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.athoracsur.2007.05.095"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2012.09.021"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e3181943c76"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2010.06.025"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00595-005-3015-6"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.tp.0000281918.51138.3f"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1010-7940(00)00363-8"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcvs.2010.04.009"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2011.06.005"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/ctr.12590"}],"container-title":["Clinical 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