{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:30:08Z","timestamp":1753878608212,"version":"3.41.2"},"reference-count":48,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,10,12]],"date-time":"2017-10-12T00:00:00Z","timestamp":1507766400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Transplantation"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Non\u2010<jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content> antibodies against human endothelial progenitor cells (<jats:styled-content style=\"fixed-case\">EPC<\/jats:styled-content>) in pre\u2010transplant recipient serum can have a deleterious influence on the graft. <jats:styled-content style=\"fixed-case\">EPC<\/jats:styled-content> enriched from peripheral blood have been commonly used for <jats:styled-content style=\"fixed-case\">EPC<\/jats:styled-content> cross\u2010matching. In the present study, we describe cross\u2010matches using <jats:styled-content style=\"fixed-case\">EPC<\/jats:styled-content> enriched from fresh or frozen\u2010thawed spleen cell preparations, thereby widening the sample source for deceased\u2010donor cross\u2010matching and retrospective studies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">EPC<\/jats:styled-content> cross\u2010matches were performed retrospectively using spleen cells and the flow cytometric <jats:styled-content style=\"fixed-case\">XM<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ONE<\/jats:styled-content> cross\u2010match test kit.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Healthy controls (n\u00a0=\u00a028) showed no IgG antibodies against <jats:styled-content style=\"fixed-case\">EPC<\/jats:styled-content>. When sera of 11 random dialysis patients were studied, 2 patients (18%) exhibited IgG <jats:styled-content style=\"fixed-case\">EPC<\/jats:styled-content> antibodies. When pre\u2010transplant sera of 20 kidney graft recipients with good long\u2010term graft outcome (serum creatinine 1.0\u00a0\u00b1\u00a00.2\u00a0mg\/dL measured 2463\u00a0\u00b1\u00a0324\u00a0days post\u2010transplant) were investigated using frozen\u2010thawed and then separated Tie\u20102\u2010enriched spleen cells of the original transplant donor, 3 patients (15%) had pre\u2010transplant IgG <jats:styled-content style=\"fixed-case\">EPC<\/jats:styled-content> antibodies. When pre\u2010transplant sera of 5 patients with intra\u2010operative graft loss were studied employing the original donor spleen cells, 4 (80%) patients showed IgG <jats:styled-content style=\"fixed-case\">EPC<\/jats:styled-content> antibodies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Cross\u2010matches with spleen cell\u2010derived <jats:styled-content style=\"fixed-case\">EPC<\/jats:styled-content> using the <jats:styled-content style=\"fixed-case\">XM<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ONE<\/jats:styled-content> assay are technically possible. Our very preliminary experience suggests clinical relevance.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ctr.13118","type":"journal-article","created":{"date-parts":[[2017,9,19]],"date-time":"2017-09-19T10:22:45Z","timestamp":1505816565000},"source":"Crossref","is-referenced-by-count":1,"title":["Endothelial precursor cell cross\u2010match using Tie\u20102\u2010enriched spleen cells"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8345-1031","authenticated-orcid":false,"given":"Volker","family":"Daniel","sequence":"first","affiliation":[{"name":"Transplantation\u2010Immunology Institute of Immunology University\u2010Hospital Heidelberg Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caner","family":"S\u00fcsal","sequence":"additional","affiliation":[{"name":"Transplantation\u2010Immunology Institute of Immunology University\u2010Hospital Heidelberg Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabine","family":"Scherer","sequence":"additional","affiliation":[{"name":"Transplantation\u2010Immunology Institute of Immunology University\u2010Hospital Heidelberg Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hien","family":"Tran","sequence":"additional","affiliation":[{"name":"Transplantation\u2010Immunology Institute of Immunology University\u2010Hospital Heidelberg Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petra","family":"Gombos","sequence":"additional","affiliation":[{"name":"Transplantation\u2010Immunology Institute of Immunology University\u2010Hospital Heidelberg Heidelberg Germany"},{"name":"Department of Surgery University\u2010Hospital Heidelberg Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karina","family":"Trojan","sequence":"additional","affiliation":[{"name":"Transplantation\u2010Immunology Institute of Immunology University\u2010Hospital Heidelberg Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0994-895X","authenticated-orcid":false,"given":"Mahmoud","family":"Sadeghi","sequence":"additional","affiliation":[{"name":"Transplantation\u2010Immunology Institute of Immunology University\u2010Hospital Heidelberg Heidelberg Germany"},{"name":"Department of Surgery University\u2010Hospital Heidelberg Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Morath","sequence":"additional","affiliation":[{"name":"Department of Nephrology Center for Internal Medicine University\u2010Hospital Heidelberg Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerhard","family":"Opelz","sequence":"additional","affiliation":[{"name":"Transplantation\u2010Immunology Institute of Immunology University\u2010Hospital Heidelberg Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,10,12]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-200212150-00001"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e31823ec0ec"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.2009.01439.x"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/ctr.12665"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.6002\/ect.mesot2014.P77"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2011.03955.x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-3274(97)80016-0"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2013121277"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2013.06.005"},{"key":"e_1_2_10_11_1","first-page":"535","article-title":"A case of acute vascular rejection caused by endothelial\u2010reactive non\u2010HLA antibodies","volume":"22","author":"Holgersson J","year":"2006","journal-title":"Clin Transplant"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e3181949d4e"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2010.12.040"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e31821b60e9"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2013.06.003"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0127861"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2011060596"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2010111183"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00250.2011"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2013.05.002"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2011.03.059"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.04451007"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0198-8859(83)90006-X"},{"issue":"1","key":"e_1_2_10_24_1","first-page":"711","article-title":"Humoral rejection in two HLA identical living related donor kidney transplantsant","volume":"21","author":"Kalil J","year":"1989","journal-title":"Transplant Proc"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)66458-6"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2008.07.005"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2014.09.029"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.12763"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.12395"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.12397"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2013.07.001"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/tri.12371"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000001199"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000001231"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00505"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2016.88"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.102.19.2426"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gft488"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2007.01799.x"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/ctr.12175"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.12130"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e31827a19cc"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.12332"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/845040"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000000672"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-0012.2001.00005.x"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1434429100"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200107)44:7<1484::AID-ART269>3.0.CO;2-Q"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.TP.0000436927.08026.a8"}],"container-title":["Clinical Transplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fctr.13118","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ctr.13118","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T21:43:37Z","timestamp":1695505417000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ctr.13118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10,12]]},"references-count":48,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["10.1111\/ctr.13118"],"URL":"https:\/\/doi.org\/10.1111\/ctr.13118","archive":["Portico"],"relation":{},"ISSN":["0902-0063","1399-0012"],"issn-type":[{"type":"print","value":"0902-0063"},{"type":"electronic","value":"1399-0012"}],"subject":[],"published":{"date-parts":[[2017,10,12]]},"article-number":"e13118"}}