{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T17:36:03Z","timestamp":1778434563612,"version":"3.51.4"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,2,21]],"date-time":"2019-02-21T00:00:00Z","timestamp":1550707200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100010878","name":"AbbVie Biotherapeutics","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010878","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Transplantation"],"published-print":{"date-parts":[[2019,3]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>We investigated circulating levels of individual soluble urokinase plasminogen activation receptor (suPAR<\/jats:styled-content>) forms to determine if specific circulating fragments of suPAR<\/jats:styled-content> (II<\/jats:styled-content>\u2010III<\/jats:styled-content>) and (I) can better serve as clinical biomarkers for focal segmental glomerulosclerosis (FSGS<\/jats:styled-content>) and the risk of recurrence after transplantation.<\/jats:p><\/jats:sec>Materials and Methods<\/jats:title>Serum levels of intact suPAR<\/jats:styled-content> and its cleaved forms were measured with two assays, ELISA<\/jats:styled-content> and TR<\/jats:styled-content>\u2010FIA<\/jats:styled-content>.<\/jats:p><\/jats:sec>Results<\/jats:title>suPAR<\/jats:styled-content> levels in healthy controls were significantly lower than those who had glomerular diseases but were not significantly different between FSGS<\/jats:styled-content> patients and glomerular controls. Intact suPAR<\/jats:styled-content> (I\u2010II<\/jats:styled-content>\u2010III<\/jats:styled-content>) levels were noted to be elevated in glomerular diseases including FSGS<\/jats:styled-content>. uPAR<\/jats:styled-content> fragment (I) levels measured with the TR<\/jats:styled-content>\u2010FIA<\/jats:styled-content> 4 assay were significantly higher in FSGS<\/jats:styled-content> (695.4\u00a0+\u00a091.29\u00a0pMol\/L) than glomerular controls (239.1\u00a0+\u00a040.45\u00a0pMol\/L, P<\/jats:italic>\u00a0=\u00a00.001). However, suPAR<\/jats:styled-content>(I) levels were not significantly different between recurrent FSGS<\/jats:styled-content> and nonrecurrent FSGS<\/jats:styled-content> patients.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Our analysis of suPAR<\/jats:styled-content> using the ELISA<\/jats:styled-content> assay used in all previous studies does not appear to be a useful marker for FSGS<\/jats:styled-content> nor serve as a predictor for its recurrence after transplantation. The TR<\/jats:styled-content>\u2010FIA<\/jats:styled-content> assay results suggest that uPAR<\/jats:styled-content>(I) is a potential biomarker for FSGS<\/jats:styled-content> but not of its recurrence.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ctr.13487","type":"journal-article","created":{"date-parts":[[2019,1,28]],"date-time":"2019-01-28T22:10:02Z","timestamp":1548713402000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Identifying a potential biomarker for primary focal segmental glomerulosclerosis and its association with recurrence after transplantation"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8871-1859","authenticated-orcid":false,"given":"Efrat","family":"Harel","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Chemistry University of California San Francisco California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2505-6372","authenticated-orcid":false,"given":"Jun","family":"Shoji","sequence":"additional","affiliation":[{"name":"Kidney Transplant Service University of California San Francisco California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3150-9638","authenticated-orcid":false,"given":"Vivek","family":"Abraham","sequence":"additional","affiliation":[{"name":"AbbVie Renal Discovery Chicago Illinois"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7494-507X","authenticated-orcid":false,"given":"Loan","family":"Miller","sequence":"additional","affiliation":[{"name":"AbbVie Renal Discovery Chicago Illinois"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0511-8764","authenticated-orcid":false,"given":"Zoltan","family":"Laszik","sequence":"additional","affiliation":[{"name":"Department of Pathology University of California San Francisco California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3758-9990","authenticated-orcid":false,"given":"Tine","family":"Thurison","sequence":"additional","affiliation":[{"name":"The Finsen Laboratory Copenhagen University Hospital\/Biotech Research & Innovation Centre Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5282-3186","authenticated-orcid":false,"given":"Andrew","family":"King","sequence":"additional","affiliation":[{"name":"AbbVie Renal Discovery Chicago Illinois"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8998-4514","authenticated-orcid":false,"given":"Adam","family":"Olshen","sequence":"additional","affiliation":[{"name":"Helen Diller Family Comprehensive Cancer Center University of California San Francisco California"},{"name":"Department of Epidemiology and Biostatistics University of California San Francisco California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2528-9555","authenticated-orcid":false,"given":"Joey","family":"Leung","sequence":"additional","affiliation":[{"name":"Kidney Transplant Service University of California San Francisco California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0493-8437","authenticated-orcid":false,"given":"Gyula","family":"Szabo","sequence":"additional","affiliation":[{"name":"Department of Pathology University of California San Francisco California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0715-5166","authenticated-orcid":false,"given":"Byron","family":"Hann","sequence":"additional","affiliation":[{"name":"Helen Diller Family Comprehensive Cancer Center University of California San Francisco California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1007-1585","authenticated-orcid":false,"given":"Gunilla","family":"H\u00f8yer\u2010Hansen","sequence":"additional","affiliation":[{"name":"Department of Pathology University of California San Francisco California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7704-9185","authenticated-orcid":false,"given":"Charles S.","family":"Craik","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry University of California San Francisco California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6701-4680","authenticated-orcid":false,"given":"Flavio","family":"Vincenti","sequence":"additional","affiliation":[{"name":"Kidney Transplant Service University of California San Francisco California"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,2,21]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2012030302"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4242"},{"issue":"3","key":"e_1_2_5_4_1","doi-asserted-by":"crossref","first-page":"1926","DOI":"10.1016\/S0021-9258(18)52382-6","article-title":"Cellular receptor for urokinase plasminogen activator Carboxyl\u2010terminal processing and membrane anchoring by glycosyl\u2010phosphatidylinositol","volume":"266","author":"Ploug M","year":"1991","journal-title":"J Biol Chem"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm977"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2423(07)44003-3"},{"issue":"3","key":"e_1_2_5_7_1","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1042\/bj3580673","article-title":"Urokinase\u2010catalysed cleavage of the urokinase receptor requires an intact glycolipid anchor","volume":"358","author":"Hoyer\u2010Hansen G","year":"2001","journal-title":"Biochem J"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2821"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2010.144410"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1696"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2013040425"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2014.346"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2004.038232"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2004.11.020"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-08-0096"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2411"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1506362"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2014.113"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2013.544"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2013.549"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2013.505"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2012.32"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-013-2452-5"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e3182977ab1"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2013.204"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1155\/2009\/504294"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMe1512997"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1515787"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.h5894"},{"issue":"10","key":"e_1_2_5_30_1","doi-asserted-by":"crossref","first-page":"1868","DOI":"10.1093\/clinchem\/43.10.1868","article-title":"ELISA determination of soluble urokinase receptor in blood from healthy donors and cancer patients","volume":"43","author":"Stephens RW","year":"1997","journal-title":"Clin Chem"}],"container-title":["Clinical Transplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fctr.13487","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ctr.13487","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ctr.13487","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ctr.13487","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T08:16:47Z","timestamp":1694333807000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ctr.13487"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2,21]]},"references-count":29,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,3]]}},"alternative-id":["10.1111\/ctr.13487"],"URL":"https:\/\/doi.org\/10.1111\/ctr.13487","archive":["Portico"],"relation":{},"ISSN":["0902-0063","1399-0012"],"issn-type":[{"value":"0902-0063","type":"print"},{"value":"1399-0012","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,2,21]]},"assertion":[{"value":"2018-08-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-01-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13487"}}