{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T22:40:30Z","timestamp":1788043230843,"version":"build-2784847793"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000032","name":"Institute of Infection and Immunity","doi-asserted-by":"publisher","award":["102683"],"award-info":[{"award-number":["102683"]}],"id":[{"id":"10.13039\/501100000032","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Transplantation"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>As a step toward evaluating the association between Epstein\u2010Barr virus genetic diversity and post\u2010transplant lymphoproliferative disorder (PTLD), we conducted a preliminary study to compare the genetic diversity of the EBNA\u20101 gene among transplant patients and patients with infectious mononucleosis (IM).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We sequenced the EBNA\u20101 gene in blood samples from study subjects using Sanger methodology. The sequences were aligned with a reference strain and compared with publicly available sequences.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We analyzed 33 study samples and 25 publicly available sequences along with the reference strain B95\u20108. The evaluable samples were from sixteen patients with IM (median age 14.0\u00a0years, range 2\u201024) and 17 transplant patients. There were six children without PTLD (median age 1.93\u00a0years, range 0.79\u20107.46) and 11 who developed PTLD (median age 5.67\u00a0years, range 0.96\u201017.45). A predominant EBNA\u20101 variant (P\u2010thr) was identified across the study groups. Differences were observed between the samples from the IM patients compared with the transplant samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The predominant EBNA\u20101 strain is in contrast to reports of the predominant strain in North America. The results suggest differences between the EBNA\u20101 strains among the study groups. Further studies will examine the relationship between EBNA\u20101 strains and PTLD occurrence and outcomes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ctr.13504","type":"journal-article","created":{"date-parts":[[2019,2,21]],"date-time":"2019-02-21T06:33:15Z","timestamp":1550730795000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Epstein\u2010Barr virus latent gene EBNA\u20101 genetic diversity among transplant patients compared with patients with infectious mononucleosis"],"prefix":"10.1111","volume":"33","author":[{"given":"Katie","family":"Sullivan","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Hospital for Sick Children University of Toronto Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Isabel","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Hospital for Sick Children University of Toronto Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nasser","family":"Khodai\u2010Booran","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Hospital for Sick Children University of Toronto Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tara A.","family":"Paton","sequence":"additional","affiliation":[{"name":"The Research Institute, Hospital for Sick Children University of Toronto Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Abdulnoor","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Hospital for Sick Children University of Toronto Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6323-7222","authenticated-orcid":false,"given":"Anne I.","family":"Dipchand","sequence":"additional","affiliation":[{"name":"The Transplant and Regenerative Medicine Centre, Hospital for Sick Children University of Toronto Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2547-3846","authenticated-orcid":false,"given":"Diane","family":"H\u00e9bert","sequence":"additional","affiliation":[{"name":"The Transplant and Regenerative Medicine Centre, Hospital for Sick Children University of Toronto Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9998-5692","authenticated-orcid":false,"given":"Vicky L.","family":"Ng","sequence":"additional","affiliation":[{"name":"The Transplant and Regenerative Medicine Centre, Hospital for Sick Children University of Toronto Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2326-7731","authenticated-orcid":false,"given":"Upton D.","family":"Allen","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Hospital for Sick Children University of Toronto Toronto Ontario Canada"},{"name":"The Transplant and Regenerative Medicine Centre, Hospital for Sick Children University of Toronto Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/310207a0"},{"key":"e_1_2_8_3_1","first-page":"19922015","volume-title":"Textbook of Pediatric Infectious","author":"Leach CT","year":"2014"},{"key":"e_1_2_8_4_1","first-page":"45","article-title":"Epstein\u2010Barr virus strain variation","volume":"390","author":"Farrell PJ","year":"2015","journal-title":"Curr Top Microbiol Immunol"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2009.07.005"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/v5010226"},{"key":"e_1_2_8_7_1","first-page":"3","article-title":"EBNA1","volume":"391","author":"Frappier L","year":"2015","journal-title":"Curr Top Microbiol Immunol"},{"key":"e_1_2_8_8_1","first-page":"177","article-title":"Variation in the sequence of Epstein Barr virus nuclear antigen 1 in normal peripheral blood lymphocytes and in Burkitt's lymphomas","volume":"13","author":"Bhatia K","year":"1996","journal-title":"Oncogene"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-78-7-1663"},{"key":"e_1_2_8_10_1","unstructured":"https:\/\/www.qiagen.com\/us\/shop\/automated-solutions\/pcr-instruments\/rotor-gene-q\/?cmpxml:id=QVenILG1509rotorgeneQ_brand_GA_SEM_&kwxml:id=rotor%20gene&clear=true#orderinginformation. 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