{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T21:53:56Z","timestamp":1775858036769,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,8,2]],"date-time":"2020-08-02T00:00:00Z","timestamp":1596326400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["16SDG31080002"],"award-info":[{"award-number":["16SDG31080002"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007217","name":"Health Services Research and Development","doi-asserted-by":"publisher","award":["I01 BX003859"],"award-info":[{"award-number":["I01 BX003859"]}],"id":[{"id":"10.13039\/100007217","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Transplantation"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>While the donation after circulatory death (DCD) heart transplantation is an emerging clinical practice, the primary source of donor hearts for transplantation remains donation after brain death (DBD) donors. DCD process induces formation of NOD\u2010like receptor family pyrin domain containing\u20103 (NLRP3) inflammasome, a key mediator of inflammation\u2010driven damage to heart. Inhibition of NLRP3 inflammasome formation could be protective to DCD hearts. Five groups (n\u00a0=\u00a08 each) of mice were studied\u2014control beating heart donor (CBD) wild\u2010type (WT), DCD WT, CBD NLRP3 knockout (KO), DCD NLRP3 KO, and DCD WT NLRP3 inhibitor group. Hearts were procured and reanimated on a Langendorff system to assess physiologic parameters and then for molecular assays. NLRP3 inhibitor (50\u00a0\u00b5mol\/L) was administered to the DCD\u2010NLRP3 inhibitor group at reanimation. Tissue NLRP3 levels were 80% higher in the DCD WT group compared with the CBD\u2010WT group. Caspase\u20101 activity was significantly elevated in DCD WT but not in KO or NLRP3 inhibitor groups. The developed pressures and\u00a0\u00b1dP\/dt were significantly impaired in the DCD WT group compared with the CBD\u2010WT group, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05, but were well preserved in DCD\u2010NLRP3 inhibitor group. The DCD process activates the NLRP3 inflammasome, contributing to myocardial damage and dysfunction. NLRP3 inflammasome inhibition limits myocardial injury and preserves DCD heart function.<\/jats:p>","DOI":"10.1111\/ctr.14044","type":"journal-article","created":{"date-parts":[[2020,7,12]],"date-time":"2020-07-12T12:37:40Z","timestamp":1594557460000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Targeting the NLRP3 inflammasome to reduce warm ischemic injury in donation after circulatory death heart"],"prefix":"10.1111","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8290-043X","authenticated-orcid":false,"given":"Mohammed","family":"Quader","sequence":"first","affiliation":[{"name":"Division of Thoracic and Cardiovascular Surgery Pauley Heart Center Virginia Commonwealth University Richmond Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eleonora","family":"Mezzaroma","sequence":"additional","affiliation":[{"name":"School of Pharmacy Virginia Commonwealth University Richmond Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristine","family":"Kenning","sequence":"additional","affiliation":[{"name":"Division of Thoracic and Cardiovascular Surgery Pauley Heart Center Virginia Commonwealth University Richmond Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1588-4019","authenticated-orcid":false,"given":"Stefano","family":"Toldo","sequence":"additional","affiliation":[{"name":"Division of Cardiology Pauley Heart Center Virginia Commonwealth University Richmond Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,8,2]]},"reference":[{"issue":"4","key":"e_1_2_6_2_1","first-page":"e29","article-title":"Heart disease and stroke statistics\u20132015 update: a report from the American Heart Association","volume":"131","author":"Mozaffarian 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