{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T11:25:21Z","timestamp":1787829921733,"version":"build-2784847793"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,11,20]],"date-time":"2020-11-20T00:00:00Z","timestamp":1605830400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007605","name":"Aarhus Universitet","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007605","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Transplantation"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Cardiac allograft vasculopathy (CAV) limits survival after heart transplantation (HTx), and the pathogenesis is not fully clarified. We aimed to investigate a wide range of biomarkers and their correlation with micro\u2010 and macrovascular CAV and major adverse cardiac events in HTx patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We evaluated 91 cardiovascular disease\u2010related proteins in 48 HTx patients using a novel proteomic panel. Patients were dichotomized according to micro\u2010 and macrovascular CAV burden determined by coronary angiography, optical coherence tomography, and <jats:sup>15<\/jats:sup>O\u2010H<jats:sub>2<\/jats:sub>O positron emission tomography imaging. Major adverse cardiac events included significant CAV progression, heart failure, treated rejection, and cardiovascular death.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found consistent differences in two proteins involved in cholesterol homeostasis: significantly increased proprotein convertase subtilisin\/kexin type 9 (PCSK9) (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) and significantly decreased paraoxonase 3 (PON3) (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). N\u2010terminal pro\u2010brain natriuretic peptide (NT\u2010proBNP) was significantly increased in patients with microvascular CAV (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) and borderline significantly increased in patients experiencing major adverse cardiac events (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.10) and patients with macrovascular CAV (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We identified consistent changes in two proteins involved in cholesterol homeostasis which may be important players in the pathogenesis of CAV: PON3 and PCSK9. NT\u2010proBNP also showed consistent changes across all groups but only reached statistical significance in patients with microvascular CAV. Our results warrant further validation in future studies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ctr.14133","type":"journal-article","created":{"date-parts":[[2020,10,31]],"date-time":"2020-10-31T04:22:07Z","timestamp":1604118127000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Micro\u2010 and macrovascular cardiac allograft vasculopathy in relation to 91 cardiovascular biomarkers in heart transplant recipients\u2014An exploratory study"],"prefix":"10.1111","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9043-8898","authenticated-orcid":false,"given":"Kamilla Pernille","family":"Bjerre","sequence":"first","affiliation":[{"name":"Department of Cardiology Aarhus University Hospital  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tor Skibsted","family":"Clemmensen","sequence":"additional","affiliation":[{"name":"Department of Cardiology Aarhus University Hospital  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steen Hvitfeldt","family":"Poulsen","sequence":"additional","affiliation":[{"name":"Department of Cardiology Aarhus University Hospital  Aarhus Denmark"},{"name":"Department of Clinical Medicine Faculty of Health Aarhus University  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne\u2010Mette","family":"Hvas","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine Faculty of Health Aarhus University  Aarhus Denmark"},{"name":"Department of Clinical Biochemistry Aarhus University Hospital  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niels Ramsing","family":"Holm","sequence":"additional","affiliation":[{"name":"Department of Cardiology Aarhus University Hospital  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik Lerkevang","family":"Grove","sequence":"additional","affiliation":[{"name":"Department of Cardiology Aarhus University Hospital  Aarhus Denmark"},{"name":"Department of Clinical Medicine Faculty of Health Aarhus University  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten","family":"Bouchelouche","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine &amp; PET Center Aarhus University Hospital  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steen Dalby","family":"Kristensen","sequence":"additional","affiliation":[{"name":"Department of Cardiology Aarhus University Hospital  Aarhus Denmark"},{"name":"Department of Clinical Medicine Faculty of Health Aarhus University  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Eiskj\u00e6r","sequence":"additional","affiliation":[{"name":"Department of Cardiology Aarhus University Hospital  Aarhus Denmark"},{"name":"Department of Clinical Medicine Faculty of Health Aarhus University  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,11,20]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2015.08.003"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2010.05.017"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2013.02.005"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.echo.2016.01.012"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.106.647149"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0228931"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmg.2016.10.021"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/heartjnl-2011-300790"},{"key":"e_1_2_10_10_1","unstructured":"ProteomicsO.Biomarkers in Olink Cardiovascular III 2019[updated 2019]. 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