{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T22:09:12Z","timestamp":1791929352257,"version":"4.3.4"},"reference-count":57,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,2,6]],"date-time":"2021-02-06T00:00:00Z","timestamp":1612569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Transplantation"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The role of donor\u2010derived cell\u2010free DNA (dd\u2010cfDNA) in screening for cardiac allograft vasculopathy (CAV) is unknown. We hypothesized that dd\u2010cfDNA correlates with CAV, markers of inflammation, and angiogenesis in stable heart transplant (HT) recipients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sixty\u2010five HT recipients \u22652\u00a0years post\u2010transplant, without recent rejection, were stratified by high (\u22650.12%) versus low levels (&lt;0.12%) of dd\u2010cfDNA. A targeted amplification, next\u2010generation sequencing assay (AlloSure<jats:sup>\u00ae<\/jats:sup>; CareDx, Inc.) was used to detect dd\u2010cfDNA. Peripheral blood inflammatory and angiogenesis markers were assessed using a multiplex immunoassay system (Bioplex<jats:sup>\u00ae<\/jats:sup>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of 65 patients, 58 patients had a known CAV status and were included. Thirty had high levels of dd\u2010cfDNA (\u22650.12%), and 28 had low levels (&lt;0.12%). CAV was present in 63% of patients with high dd\u2010cfDNA vs. 35% with low dd\u2010cfDNA (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.047). Donor\u2010specific antibodies were present in 25% of patients with high dd\u2010cfDNA vs. 3.8% in those with low dd\u2010cfDNA (<jats:italic>p\u00a0<\/jats:italic>=\u00a0.03). There were no differences in rejection episodes, inflammatory, or angiogenesis markers. Importantly, dd\u2010cfDNA levels were not different when stratified by time post\u2010transplant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Higher dd\u2010cfDNA levels were associated with CAV in stable chronic HT recipients. Further studies are warranted to investigate a possible association between dd\u2010cfDNA levels and CAV severity and whether dd\u2010cfDNA can predict CAV progression.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ctr.14206","type":"journal-article","created":{"date-parts":[[2020,12,26]],"date-time":"2020-12-26T04:24:04Z","timestamp":1608956644000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Donor\u2010derived cell\u2010free DNA is associated with cardiac allograft vasculopathy"],"prefix":"10.1111","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7908-4611","authenticated-orcid":false,"given":"Luise","family":"Holzhauser","sequence":"first","affiliation":[{"name":"Department of Cardiology University of Chicago  Chicago IL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8351-9219","authenticated-orcid":false,"given":"Kevin 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