{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T18:19:36Z","timestamp":1767896376234,"version":"3.49.0"},"reference-count":12,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2021,7,14]],"date-time":"2021-07-14T00:00:00Z","timestamp":1626220800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Transplantation"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Donor\u2010derived cell\u2010free DNA (dd\u2010cfDNA) is a marker of allograft injury in transplant recipients; however, the relationship between dd\u2010cfDNA and other clinical parameters associated with adverse allograft outcomes is not well\u2010characterized.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We performed a retrospective analysis of kidney transplant recipients from the DART cohort (ClinicalTrials.gov Identifier: NCT02424227) to evaluate the associations between eGFR decline, <jats:italic>de novo<\/jats:italic> donor\u2010specific antibodies (dnDSA), and dd\u2010cfDNA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Both elevated dd\u2010cfDNA (\u22651%) and dd\u2010cfDNA variability (\u2265.34%) in the first post\u2010transplant year were associated with decline in eGFR \u226525% in the second year (21.4%\u00a0vs. 4.1%, <jats:italic>P<\/jats:italic>\u00a0=\u00a0.005; 25%\u00a0vs. 3.6%, <jats:italic>P<\/jats:italic>\u00a0=\u00a0.002, respectively). Compared to samples from DSA negative patients, samples from patients with concurrent <jats:italic>de novo<\/jats:italic> HLA DSAs had higher dd\u2010cfDNA levels (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.0001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>Abnormalities in dd\u2010cfDNA levels are associated with clinical parameters commonly used as surrogate endpoints for adverse allograft outcomes, raising the possibility that molecular injury as characterized by dd\u2010cfDNA could help identify patients at risk of these outcomes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ctr.14402","type":"journal-article","created":{"date-parts":[[2021,6,29]],"date-time":"2021-06-29T06:46:05Z","timestamp":1624949165000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Association between dd\u2010cfDNA levels, de novo donor specific antibodies, and eGFR decline: An analysis of the DART cohort"],"prefix":"10.1111","volume":"35","author":[{"given":"Deirdre L.","family":"Sawinski","sequence":"first","affiliation":[{"name":"Renal, Electrolyte, and Hypertension Division Perelman School of Medicine University of Pennsylvania  Philadelphia Pennsylvania USA"},{"name":"Penn Transplant Institute Hospital of the University of Pennsylvania  Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shikha","family":"Mehta","sequence":"additional","affiliation":[{"name":"Section of Transplant Nephrology University of Alabama at Birmingham  Birmingham Alabama USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tarek","family":"Alhamad","sequence":"additional","affiliation":[{"name":"Division of Nephrology Department of Medicine Washington University School of Medicine  St. Louis Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan S.","family":"Bromberg","sequence":"additional","affiliation":[{"name":"Department of Surgery and Department of Microbiology and Immunology University of Maryland School of Medicine  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard","family":"Fischbach","sequence":"additional","affiliation":[{"name":"Baylor University Medical Center  Dallas Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Aeschbacher","sequence":"additional","affiliation":[{"name":"CareDx Inc.  Brisbane California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Srinka","family":"Ghosh","sequence":"additional","affiliation":[{"name":"CareDx Inc.  Brisbane California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grigoriy","family":"Shekhtman","sequence":"additional","affiliation":[{"name":"CareDx Inc.  Brisbane California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sham","family":"Dholakia","sequence":"additional","affiliation":[{"name":"CareDx Inc.  Brisbane California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel C.","family":"Brennan","sequence":"additional","affiliation":[{"name":"Division of Nephrology Johns Hopkins University School of Medicine  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emilio","family":"Poggio","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertension Cleveland Clinic  Cleveland Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roy D.","family":"Bloom","sequence":"additional","affiliation":[{"name":"Renal, Electrolyte, and Hypertension Division Perelman School of Medicine University of Pennsylvania  Philadelphia Pennsylvania USA"},{"name":"Penn Transplant Institute Hospital of the University of Pennsylvania  Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stanley C.","family":"Jordan","sequence":"additional","affiliation":[{"name":"Department of Medicine David Geffen School of Medicine at the University of California Los Angeles  Los Angeles California USA"},{"name":"Division of Nephrology Cedars\u2010Sinai Medical Center  Los Angeles California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,7,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2012.04013.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2016091034"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.15822"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000002482"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9051480"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000003240"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/849835"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.00700117"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e31827d62e3"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2019.01.723"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2018.01.018"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2015050524"}],"container-title":["Clinical Transplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ctr.14402","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ctr.14402","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ctr.14402","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T03:36:33Z","timestamp":1693193793000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ctr.14402"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,14]]},"references-count":12,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2021,9]]}},"alternative-id":["10.1111\/ctr.14402"],"URL":"https:\/\/doi.org\/10.1111\/ctr.14402","archive":["Portico"],"relation":{},"ISSN":["0902-0063","1399-0012"],"issn-type":[{"value":"0902-0063","type":"print"},{"value":"1399-0012","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7,14]]},"assertion":[{"value":"2020-12-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e14402"}}