{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:47:40Z","timestamp":1787014060135,"version":"build-2736575974"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,1,10]],"date-time":"2022-01-10T00:00:00Z","timestamp":1641772800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/N024907\/1"],"award-info":[{"award-number":["MR\/N024907\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006662","name":"National Institute on Handicapped Research","doi-asserted-by":"publisher","award":["RP\u20102017\u201008\u2010ST2\u2010002"],"award-info":[{"award-number":["RP\u20102017\u201008\u2010ST2\u2010002"]}],"id":[{"id":"10.13039\/100006662","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012295","name":"NIHR Newcastle Biomedical Research Centre","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012295","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018956","name":"NIHR Cambridge Biomedical Research Centre","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100018956","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Transplantation"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Transplantation is an effective treatment for end\u2010stage lung disease, but the donor organ shortage is a major problem. Ex\u2010vivo lung perfusion (EVLP) of extended criteria organs enables functional assessment to facilitate clinical decision\u2010making around utilization, but the molecular processes occurring during EVLP, and how they differ between more or less viable lungs, remain to be determined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We used RNA sequencing of lung tissue to delineate changes in gene expression occurring in 10 donor lungs undergoing EVLP and compare lungs that were deemed non\u2010transplantable (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a04) to those deemed transplantable (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a06) following perfusion.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We found that lungs deemed unsuitable for transplantation had increased induction of innate immune pathways and lower expression of oxidative phosphorylation related genes. Furthermore, the expression of SCGB1A1, a gene encoding an anti\u2010inflammatory secretoglobin CC10, and other club cell genes was significantly decreased in non\u2010transplantable lungs, while CHIT\u20101 was increased. Using a larger validation cohort (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a017), we confirmed that the ratio of CHIT1 and SCGB1A1 protein levels in lung perfusate have potential utility to distinguish transplantable from non\u2010transplantable lungs (AUC .81).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Together, our data identify novel biomarkers that may assist with pre\u2010transplant lung assessment, as well as pathways that may be amenable to therapeutic intervention during EVLPAQ6.<\/jats:p>\n                  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