{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T20:13:16Z","timestamp":1790107996220,"version":"4.0.1"},"reference-count":23,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,5,29]],"date-time":"2022-05-29T00:00:00Z","timestamp":1653782400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Transplantation"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>To evaluate the initial use of label\u2010free second harmonic generation (SHG) imaging with two\u2010photon excitation (2PE) auto\u2010fluorescence in multiphoton microscopy (MPM) for the quantification of collagen\/fibrosis on preimplantation biopsies of extended criteria donors (ECD).<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Twenty preimplantation core biopsies were extracted from 10 donor kidney samples, of which originated from seven donors. Kidney Donor Profile Index (KDPI) and Remuzzi scores of biopsies were calculated. Collagen parameters measured included quantification by the Collagen Area Ratio in Total Tissue (CART) and qualitative measurements by Collagen Reticulation Index (CRI).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Biopsies classified with\u00a0&gt;\u00a085% KDPI scores had significantly higher CART (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.011) and lower CRI values (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.025) than biopsies with \u2264 85% KDPI scores. Increase in CRI values correlated significantly with rise in recipient creatinine levels 1\u2010year post\u2010transplant (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.027; 95% CI: 4.635\u201066.797).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>MPM is an evolving technology that enables the quantification of the amount (CART) and quality (CRI) of collagen deposition in unstained preimplantation biopsies of donor kidneys stratified by KDPI scores. This initial evaluation found significant differences in both parameters between donor kidneys with more or less than 85% KDPI.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ctr.14717","type":"journal-article","created":{"date-parts":[[2022,5,22]],"date-time":"2022-05-22T11:21:24Z","timestamp":1653218484000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Multi\u2010photon microscopy for the evaluation of interstitial fibrosis in extended criteria donor kidneys: A proof\u2010of\u2010concept study"],"prefix":"10.1111","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2303-0750","authenticated-orcid":false,"given":"Wei Zheng","family":"So","sequence":"first","affiliation":[{"name":"Yong Loo Lin School of Medicine National University Singapore  Singapore 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