{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T14:46:20Z","timestamp":1787669180689,"version":"build-2736575974"},"reference-count":18,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,11,27]],"date-time":"2022-11-27T00:00:00Z","timestamp":1669507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Transplantation"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Immune cell function assay (ICFA) and CD3 lymphocyte counts have been considered to be useful in discerning the overall intensity of immunosuppression in pediatric orthotopic heart transplant (OHT) recipients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The aim of this retrospective analysis was to evaluate trends of ICFA and CD3 lymphocyte counts and their association with adverse outcomes post\u2010OHT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 381 ICFA and 493 CD3 laboratory values obtained in 78 patients within six months post\u2010OHT were analyzed. There were 14 patients treated for biopsy\u2010proven acute rejection, four of whom had ISHLT grade 2R\/3A rejection. In patients with rejection versus those without, CD3 and ICFA values were 122 (IQR 74.5\u2013308) cells\/mm<jats:sup>2<\/jats:sup> and 224.5 (IQR 132\u2013343.5) ng\/ml compared to 231.8 (IQR 68\u2013421) cells\/m<jats:sup>2<\/jats:sup> and 191 (IQR 81.5\u2013333) ng\/mL (<jats:italic>p<\/jats:italic>\u00a0=\u00a0NS for both). Twenty\u2010six patients had at least one detectable cytomegalovirus or Epstein\u2010Barr virus DNAemia within the study timeframe. In patients with viremia versus those without, CD3 and ICFA values were 278.5 (IQR 68\u2013552) cells\/mm<jats:sup>2<\/jats:sup> and 130 (IQR 48\u2013284) ng\/ml compared to 195 (IQR 74.5\u2013402.5) cells\/mm<jats:sup>2<\/jats:sup> and 212 (IQR 89\u2013342) ng\/ml (<jats:italic>p<\/jats:italic>\u00a0=\u00a0NS for both).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>No association was found between these immune markers and adverse outcomes. In the absence of larger pediatric studies justifying the role of these tests in identifying elevated risk profiles post OHT, we do not recommend their routine use.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ctr.14858","type":"journal-article","created":{"date-parts":[[2022,11,14]],"date-time":"2022-11-14T05:08:23Z","timestamp":1668402503000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Immune cell function assay and T lymphocyte counts lack association with rejection or infection in pediatric heart transplant recipients"],"prefix":"10.1111","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1215-7390","authenticated-orcid":false,"given":"Justin K.","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Pharmacy NewYork\u2010Presbyterian Hospital Columbia University Irving Medical Center  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5450-3995","authenticated-orcid":false,"given":"David M.","family":"Salerno","sequence":"additional","affiliation":[{"name":"Department of Pharmacy NewYork\u2010Presbyterian Hospital Weill Cornell Medical Center  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2521-2080","authenticated-orcid":false,"given":"Heather","family":"Corbo","sequence":"additional","affiliation":[{"name":"Department of Pharmacy NewYork\u2010Presbyterian Hospital Columbia University Irving Medical Center  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7700-6975","authenticated-orcid":false,"given":"Benjamin S.","family":"Mantell","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Columbia University Irving Medical Center  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5491-8085","authenticated-orcid":false,"given":"Marc","family":"Richmond","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Columbia University Irving Medical Center  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0645-8007","authenticated-orcid":false,"given":"Amy","family":"Rothkopf","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Columbia University Irving Medical Center  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9688-0879","authenticated-orcid":false,"given":"Irene D.","family":"Lytrivi","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Columbia University Irving Medical Center  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,11,27]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"ImmuKnow\u00ae Immune Cell Function.Eurofins Viracor web site. Accessed May 31 2022.https:\/\/www.eurofins\u2010viracor.com\/clinical\/test\u2010menu\/9000\u2010immuknow\/"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.7182\/pit2014664"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0012.2005.00429.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/petr.12298"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2008.10.001"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2009.12.015"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.tp.0000234837.02126.70"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e3181cbabe6"},{"key":"e_1_2_9_10_1","doi-asserted-by":"crossref","first-page":"e14745","DOI":"10.1111\/ctr.14745","article-title":"Pre\u2010transplant immune cell function assay as a predictor of early cardiac allograft rejection","author":"Maidman SD","year":"2022","journal-title":"Clin Transplant"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.31744\/einstein_journal\/2018AO4278"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/petr.12193"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-200204270-00026"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2004.11.099"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trim.2006.03.014"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2005.03.019"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-197508000-00005"},{"issue":"2","key":"e_1_2_9_18_1","first-page":"155","article-title":"Randomized clinical trial of antithymocyte globulin in cadaver renal allograft recipients: importance of T cell monitoring","volume":"80","author":"Cosimi AB","year":"1976","journal-title":"Surgery"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2008.05.014"}],"container-title":["Clinical Transplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ctr.14858","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ctr.14858","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ctr.14858","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T12:56:09Z","timestamp":1692449769000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ctr.14858"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,27]]},"references-count":18,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,2]]}},"alternative-id":["10.1111\/ctr.14858"],"URL":"https:\/\/doi.org\/10.1111\/ctr.14858","archive":["Portico"],"relation":{},"ISSN":["0902-0063","1399-0012"],"issn-type":[{"value":"0902-0063","type":"print"},{"value":"1399-0012","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,27]]},"assertion":[{"value":"2022-09-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e14858"}}