{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T19:28:46Z","timestamp":1774553326811,"version":"3.50.1"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T00:00:00Z","timestamp":1735603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Transplantation"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Early posttransplant cytomegalovirus (CMV) infections in CMV seronegative solid organ transplant recipients (SOTR) with CMV seronegative donors (D\u2212\/R\u2212) are often attributed transfusion\u2010transmitted CMV. The prevalence of false\u2010negative donor CMV serology in D\u2212\/R\u2212 SOTR with early CMV infections has not been explored.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We determined the frequency and characteristics of CMV DNAemia that occurred within 90 days of transplant among adult SOTR classified as D\u2212\/R\u2212 who underwent a first SOT at a single center between February 25, 2014 and February 25, 2024. Repeat donor CMV antibody testing was performed on stored donor sera if possible.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Thirteen of 737 (1.8%) D\u2212\/R\u2212 SOTR from 12 donors developed CMV DNAemia within 90 days of transplant (median time to DNAemia: 28 days, interquartile range 23\u201342 days). Five (38%) recipients experienced CMV disease either before (<jats:italic>n<\/jats:italic> = 2) or after (<jats:italic>n<\/jats:italic> = 3) CMV DNAemia was identified, and five (38%) developed CMV antiviral resistance mutations during their course. Repeat CMV antibody testing was performed on sera from four donors to five recipients and was positive in three (75%) tested donors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Early CMV infections in D\u2212\/R\u2212 SOTR are uncommon but associated with high morbidity. CMV transmission from organ donors with false negative CMV serology is an important source of early CMV infections in D\u2212\/R\u2212 SOTR. Clinicians should suspect and promptly report early CMV infections in D\u2212\/R\u2212 SOTR as potential donor\u2010derived processes, regardless of donor and\/or recipient transfusion histories. Reporting such cases is essential to promote broader investigations that may identify suboptimal donor CMV screening assays.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ctr.70073","type":"journal-article","created":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T13:19:11Z","timestamp":1735651151000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Contemporary Cytomegalovirus (CMV) Infections in Low\u2010Risk CMV Seronegative Recipients of Solid Organ Transplants From CMV Seronegative Donors (D\u2212\/R\u2212): Time to Reexamine Donor CMV Serostatus"],"prefix":"10.1111","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9424-1870","authenticated-orcid":false,"given":"Madeleine R.","family":"Heldman","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases Department of Medicine Duke University School of Medicine  Durham North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6411-198X","authenticated-orcid":false,"given":"Julia A.","family":"Messina","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Department of Medicine Duke University School of Medicine  Durham North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2442-7079","authenticated-orcid":false,"given":"Annette J.","family":"Schlueter","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Iowa  Iowa City Iowa USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7012-685X","authenticated-orcid":false,"given":"Mark J.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Pathology Duke University School of Medicine  Durham North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7684-431X","authenticated-orcid":false,"given":"Jennifer L.","family":"Saullo","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Department of Medicine Duke University School of Medicine  Durham North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7387-1171","authenticated-orcid":false,"given":"Rachel A.","family":"Miller","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Department of Medicine Duke University School of Medicine  Durham North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,12,31]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00043\u201019"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000002191"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/ctr.13512"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/tid.13564"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.16066"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537\u20102995.2009.02486.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12250"},{"key":"e_1_2_9_9_1","unstructured":"Guidance for Industry: Pre\u2010Storage Leukocyte Reduction of Whole Blood and Blood Components Intended for Transfusion U.S. Department of Health and Human Services Food and Drug Administration Center for Biologics Evaluation and Research 2012 accessed August 15 2024 https:\/\/www.fda.gov\/media\/84460\/download."},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.tp.0000226071.12562.1a"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbi.2014.07.006"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciae321"},{"key":"e_1_2_9_13_1","unstructured":"OPTN: Organ Procurement and Transplantation Network Policies Policy 15.5: Transplant Program Requirements for Communicating PostTransplant Discovery of Disease or Malignancy accessed September 15 2024 https:\/\/optn.transplant.hrsa.gov\/media\/eavh5bf3\/optn_policies.pdf."},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cix259"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.16178"},{"key":"e_1_2_9_16_1","unstructured":"OPTN: Organ Procurement and Transplantation Network Policies Policy 2.9: Required Deceased Donor Infectious Disease Testing accessed June 27 2024.https:\/\/optn.transplant.hrsa.gov\/media\/eavh5bf3\/optn_policies.pdf."},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/ctr.15301"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537\u20102995.2011.03203.x"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciad550"},{"key":"e_1_2_9_20_1","unstructured":"Immucor Inc.Capture\u2010CMV Solid Phase System for Detection of IgG and IgM Antibodies to Cytomegalovirus (package insert) 2019 accessed November 5 2024 https:\/\/cdnmedia.eurofins.com\/eurofins\u2010us\/media\/12161187\/capture\u2010cmv\u2010neo\u2010iris.pdf."},{"key":"e_1_2_9_21_1","unstructured":"IIC\u2010CSPSfDoIaIAtCpiAN https:\/\/www.fda.gov\/media\/74074\/download."}],"container-title":["Clinical Transplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ctr.70073","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,26]],"date-time":"2025-01-26T11:36:18Z","timestamp":1737891378000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ctr.70073"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,31]]},"references-count":20,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1111\/ctr.70073"],"URL":"https:\/\/doi.org\/10.1111\/ctr.70073","archive":["Portico"],"relation":{},"ISSN":["0902-0063","1399-0012"],"issn-type":[{"value":"0902-0063","type":"print"},{"value":"1399-0012","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,31]]},"assertion":[{"value":"2024-10-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70073"}}