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However, transplant\u2010related complications, including hemorrhagic cystitis (HC), remain major causes\u00a0of adverse outcomes and decreased survival. This study examines the associations of HC with pharmacogenetic single nucleotide polymorphisms (SNPs) and clinical characteristics.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This retrospective study included 259 pediatric patients who underwent allo\u2010HSCT. HC was predefined as the primary endpoint, and other major complications were analyzed as secondary exploratory outcomes. Bone marrow samples were collected at initial diagnosis for genomic DNA extraction. SNP genotyping was performed by targeted next\u2010generation sequencing via a customized 30\u2010gene panel.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Among the 30 SNPs analyzed, all except\n                      <jats:italic>CYP2C19<\/jats:italic>\n                      (c.99T &gt; C) were in Hardy\u2010Weinberg equilibrium (all\n                      <jats:italic>p<\/jats:italic>\n                      &gt; 0.05). In univariate Cox regression,\n                      <jats:italic>CYP2C19<\/jats:italic>\n                      (c.681G &gt; A) GA\/AA genotype, older age, higher body weight, haploidentical transplantation, use of cord blood in the graft, peri\u2010engraftment syndrome (peri\u2010ES), severe acute graft\u2010versus\u2010host disease, and cytomegalovirus infection were significantly associated with an increased risk of HC, whereas HLA 9\/10\u201010\/10 match appeared protective (all\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05). Multivariate Cox analysis identified\n                      <jats:italic>CYP2C19<\/jats:italic>\n                      (c.681G &gt; A) GA\/AA (hazard ratio (HR) = 1.90,\n                      <jats:italic>p<\/jats:italic>\n                      = 0.021), older age (\n                      <jats:italic>HR<\/jats:italic>\n                      = 2.14,\n                      <jats:italic>p<\/jats:italic>\n                      = 0.036), and peri\u2010ES (\n                      <jats:italic>HR<\/jats:italic>\n                      = 1.86,\n                      <jats:italic>p<\/jats:italic>\n                      = 0.034) as independent risk factors for HC.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      This study demonstrates that\n                      <jats:italic>CYP2C19<\/jats:italic>\n                      (c.681G &gt; A) GA\/AA genotype, older age, and peri\u2010ES are each independent risk factors for HC after allo\u2010HSCT. These findings highlight the value of pharmacogenetic and clinical profiling in predicting susceptibility to HC and guiding personalized preventive strategies, contributing to a deeper understanding of the interplay among genetic predisposition, immune responses, and chemotherapy\u2010related toxicity in HC pathogenesis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ctr.70474","type":"journal-article","created":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T07:34:12Z","timestamp":1770708852000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Association of\n                    <i>CYP2C19<\/i>\n                    Single Nucleotide Polymorphism With Hemorrhagic Cystitis After Pediatric Allogeneic Hematopoietic Stem Cell Transplantation"],"prefix":"10.1111","volume":"40","author":[{"given":"Weiliang","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of 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