{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:54:27Z","timestamp":1790841267784,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Transplantation"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Hypothermic oxygenated machine perfusion (HOPE) is a simple ex\u2010situ treatment for liver grafts. However, it lacks viability assessment capabilities. Flavin mononucleotide (FMN) and D\u2010dimer may be useful biomarkers of graft quality during HOPE. This exploratory study aimed to determine whether effluent FMN and D\u2010dimer concentrations were associated with outcomes in liver transplants treated with HOPE. This is a retrospective, single\u2010center study of all grafts treated with HOPE in New Zealand from 2022\u20102025. Fifty\u2010four grafts were treated with HOPE. Effluent concentrations of FMN (\n                    <jats:italic>n<\/jats:italic>\n                    = 30) and D\u2010dimer (\n                    <jats:italic>n<\/jats:italic>\n                    = 24) were analyzed. Post\u2010transplant cholangiopathy (PTxC) with graft loss occurred in five recipients of donation after circulatory death grafts. The median summation of FMN concentrations taken over the first two hours of perfusion was higher in the PTxC group (four PTxC events among the 30 grafts with FMN measurements;\n                    <jats:italic>p<\/jats:italic>\n                    = 0.034). Decision curve analysis suggested that summated FMN models may add net benefit over default strategies, particularly in DCD grafts, although with only five outcome events these analyses do not define implantation thresholds. D\u2010dimer concentrations were not significantly different in PTxC grafts (\n                    <jats:italic>p<\/jats:italic>\n                    &gt; 0.05). Whilst perfusate FMN has promise as a predictor of PTxC in HOPE grafts, especially if summated over several time points, prospective external validation is required before it could contribute to transplantation decision\u2010making.\n                  <\/jats:p>","DOI":"10.1111\/ctr.70697","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:54:10Z","timestamp":1790837650000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Perfusate Flavin Mononucleotide and D\u2010Dimer as Viability Biomarkers in Australasia's First Hypothermic Machine Perfusion Programme for Liver Transplantation"],"prefix":"10.1111","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1790-6699","authenticated-orcid":false,"given":"Jacques","family":"Eastment","sequence":"first","affiliation":[{"name":"New Zealand Liver Transplant Unit Auckland City Hospital  Auckland New Zealand"},{"name":"Department of Surgery, School of Medicine The University of Queensland  Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5280-3871","authenticated-orcid":false,"given":"Jules","family":"Devaux","sequence":"additional","affiliation":[{"name":"Applied Surgery and Metabolism Laboratory University of Auckland  Auckland New Zealand"},{"name":"Institute For Molecular Bioscience The University of Queensland  Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3801-673X","authenticated-orcid":false,"given":"Kevin","family":"Yi","sequence":"additional","affiliation":[{"name":"New Zealand Liver Transplant Unit Auckland City Hospital  Auckland New Zealand"},{"name":"Department of Surgery, School of Medicine University of Auckland  Auckland New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Swan","sequence":"additional","affiliation":[{"name":"New Zealand Liver Transplant Unit Auckland City Hospital  Auckland New Zealand"},{"name":"Department of Surgery, School of Medicine University of Auckland  Auckland New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8667-3449","authenticated-orcid":false,"given":"John","family":"McCall","sequence":"additional","affiliation":[{"name":"New Zealand Liver Transplant Unit Auckland City Hospital  Auckland New Zealand"},{"name":"Department of Surgery, School of Medicine University of Auckland  Auckland New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1324-703X","authenticated-orcid":false,"given":"Andrew","family":"Jones","sequence":"additional","affiliation":[{"name":"Queensland Digital Health Centre The University of Queensland  Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7759-5338","authenticated-orcid":false,"given":"Louise","family":"Barbier","sequence":"additional","affiliation":[{"name":"New Zealand Liver Transplant Unit Auckland City Hospital  Auckland New Zealand"},{"name":"Department of Surgery, School of Medicine University of Auckland  Auckland New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajt.2024.03.013"},{"issue":"1","key":"e_1_2_14_3_1","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1097\/LVT.0000000000000270","article-title":"Discarded Livers Tested by Normothermic Machine Perfusion in the VITTAL Trial: Secondary End Points and 5\u2010Year Outcomes","volume":"30","author":"Mergental H.","year":"2024","journal-title":"Liver Transplantation"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/lvt.0000000000000567"},{"key":"e_1_2_14_5_1","doi-asserted-by":"crossref","first-page":"35","DOI":"10.57603\/EJT-007","article-title":"Hypothermic Machine Perfusion of the Liver. The Reasons for Success","volume":"1","author":"Del Prete L.","year":"2023","journal-title":"European Journal of Transplantation"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.10515"},{"key":"e_1_2_14_7_1","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.ijsu.2020.04.057","article-title":"Hypothermic Machine Perfusion in Liver Transplantation","volume":"82","author":"Karangwa S.","year":"2020","journal-title":"International Journal of Surgery"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2023.05.027"},{"key":"e_1_2_14_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2031532"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2022.12.030"},{"key":"e_1_2_14_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/bjs\/znae321"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/sla.0000000000003513"},{"key":"e_1_2_14_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2024.08.030"},{"issue":"4","key":"e_1_2_14_14_1","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1097\/LVT.0000000000000512","article-title":"Validation of Mitochondrial FMN as a Predictor for Early Allograft Dysfunction and Patient Survival Measured During Hypothermic Oxygenated Perfusion","volume":"31","author":"Dingfelder J.","year":"2025","journal-title":"Liver Transplantation"},{"key":"e_1_2_14_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/tp.0000000000004475"},{"key":"e_1_2_14_16_1","doi-asserted-by":"publisher","DOI":"10.4103\/sja.SJA_543_18"},{"key":"e_1_2_14_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2017.10.034"},{"key":"e_1_2_14_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2020.09.015"},{"key":"e_1_2_14_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0b013e318296c732"},{"key":"e_1_2_14_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0b013e3182365081"},{"key":"e_1_2_14_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.csda.2010.11.018"},{"key":"e_1_2_14_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/0272989x06295361"},{"key":"e_1_2_14_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/bimj.201800240"},{"key":"e_1_2_14_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472\u20106947\u20108\u201053"},{"key":"e_1_2_14_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2020.103014"},{"key":"e_1_2_14_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0079097"},{"key":"e_1_2_14_27_1","doi-asserted-by":"publisher","DOI":"10.1097\/sla.0000000000006876"},{"key":"e_1_2_14_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40472\u2010025\u201000467\u20107"}],"container-title":["Clinical Transplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ctr.70697","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ctr.70697","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ctr.70697","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:54:11Z","timestamp":1790837651000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ctr.70697"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,30]]},"references-count":27,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/ctr.70697"],"URL":"https:\/\/doi.org\/10.1111\/ctr.70697","archive":["Portico"],"relation":{},"ISSN":["0902-0063","1399-0012"],"issn-type":[{"value":"0902-0063","type":"print"},{"value":"1399-0012","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,30]]},"assertion":[{"value":"2025-12-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70697"}}