{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T15:44:29Z","timestamp":1791906269970,"version":"4.3.4"},"reference-count":49,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:00:00Z","timestamp":1791849600000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100010915","name":"American Society of Transplant Surgeons","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010915","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Transplantation"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To determine whether Raman spectroscopy\u2010based urine molecular fingerprinting (UMF) may predict delayed graft function (DGF) and assess renal allograft quality prior to transplant and monitor its state of health after transplantation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Current methods for assessing the quality of the renal allograft at baseline and its state of health post\u2010transplantation are inadequate.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We conducted a pilot study in 26 patients receiving DCD allografts. UMF was performed on urine samples collected from study participants immediately before transplantation (baseline), on postoperative day 1\u20133, and monthly thereafter. UMF findings were correlated with clinical and laboratory data. Primary endpoint was identification of a DGF fingerprint. Secondary endpoints were identification of fingerprints for acute rejection, CMV, BK and eGFR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      DGF was observed in 58% of our patients. UMF fingerprints from patients with DGF were distinct from UMF fingerprints of non\u2010DGF patients. Notably, UMF analysis of baseline urine samples from patients\n                      <jats:italic>prior to<\/jats:italic>\n                      transplantation also predicted development of DGF. A correlation of UMF with eGFR was observed, whereas we did not identify fingerprints specific for rejection, CMV or BK possibly due to the small patient population.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>In this exploratory study, UMF predicts DGF prior to transplantation and provides information on the state of health of the allograft following transplantation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ctr.70716","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T14:45:31Z","timestamp":1791902731000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Raman Urine Fingerprinting to Predict DGF, Assess Renal Allograft Quality, and Monitor Its State of Health"],"prefix":"10.1111","volume":"40","author":[{"given":"Giuseppe","family":"Orlando","sequence":"first","affiliation":[{"name":"Department of Surgery Section of Transplantation Atrium Wake Forest Baptist Medical Center Wake Forest School of Medicine  Winston\u2010Salem North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan S.","family":"Senger","sequence":"additional","affiliation":[{"name":"Department of Biological Systems Engineering College of Agriculture and Life Sciences and College of Engineering Virginia Tech  Blacksburg Virginia USA"},{"name":"Rametrix Technologies, Inc.  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E.","family":"McCracken","sequence":"additional","affiliation":[{"name":"Department of Surgery Section of Transplantation Atrium Wake Forest Baptist Medical Center Wake Forest School of Medicine  Winston\u2010Salem North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan C.","family":"Farney","sequence":"additional","affiliation":[{"name":"Department of Surgery Section of Transplantation Atrium Wake Forest Baptist Medical Center Wake Forest School of Medicine  Winston\u2010Salem North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert J.","family":"Stratta","sequence":"additional","affiliation":[{"name":"Department of Surgery Section of Transplantation Atrium Wake Forest Baptist Medical Center Wake Forest School of Medicine  Winston\u2010Salem North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher J.","family":"Webb","sequence":"additional","affiliation":[{"name":"Department of 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