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Here, we characterized the effect of \u03b2\u2010adrenergic stress in familial dilated cardiomyopathy (DCM) using human\u2010induced pluripotent stem cell (hiPSC)\u2010derived cardiomyocytes (CMs) from a patient with <jats:italic>RBM20<\/jats:italic> DCM. Our findings suggest that \u03b2\u2010adrenergic stimulation accelerated defective Ca<jats:sup>2+<\/jats:sup> homeostasis, apoptotic changes, and sarcomeric disarray in familial DCM hiPSC\u2010CMs. Furthermore, pharmacological modulation of abnormal Ca<jats:sup>2+<\/jats:sup> handling by pretreatment with \u03b2\u2010blocker, carvedilol, or Ca<jats:sup>2+<\/jats:sup>\u2010channel blocker, verapamil, significantly decreased the area under curve, reduced percentage of disorganized cells, and decreased terminal deoxynucleotidyl transferase\u2010mediated deoxyuridine triphosphate nick\u2010end labeling (TUNEL)\u2010positive apoptotic loci in familial DCM hiPSC\u2010CMs after \u03b2\u2010adrenergic stimulation. These translational data provide patient\u2010based <jats:italic>in vitro<\/jats:italic> analysis of \u03b2\u2010adrenergic stress in <jats:italic>RBM20<\/jats:italic>\u2010deficient familial DCM hiPSC\u2010CMs and evaluation of therapeutic interventions to modify heart disease progression, which may be personalized, but more importantly generalized in the clinic.<\/jats:p>","DOI":"10.1111\/cts.12393","type":"journal-article","created":{"date-parts":[[2016,4,22]],"date-time":"2016-04-22T22:51:06Z","timestamp":1461365466000},"page":"158-167","source":"Crossref","is-referenced-by-count":38,"title":["Pharmacological Modulation of Calcium Homeostasis in Familial Dilated Cardiomyopathy: An <i>In Vitro<\/i> Analysis From an RBM20 Patient\u2010Derived iPSC Model"],"prefix":"10.1111","volume":"9","author":[{"given":"SP","family":"Wyles","sequence":"first","affiliation":[{"name":"Center for Clinical and Translational Sciences, Mayo Clinic  Rochester Minnesota USA"},{"name":"Center for 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