{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T21:52:45Z","timestamp":1757454765348},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,12,21]],"date-time":"2019-12-21T00:00:00Z","timestamp":1576886400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007847","name":"Natural Science Foundation of Jilin Province","doi-asserted-by":"publisher","award":["20190201212JC"],"award-info":[{"award-number":["20190201212JC"]}],"id":[{"id":"10.13039\/100007847","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["ascpt.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Translational Sci"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:p>Nasopharyngeal carcinoma (<jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content>) is a common cancer found in the nasopharynx with high metastatic and invasive nature. Increasing evidences have identified the critical role of gene therapy in <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content> treatment. Hence, this study was designed to identify specific gene markers that affected <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content> progression through gene expression profile analysis. <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content>\u2010related gene expression data\u00a0set gene set enrichment (<jats:styled-content style=\"fixed-case\">GSE<\/jats:styled-content>)53819 were retrieved and analyzed to screen out differentially expressed genes (<jats:styled-content style=\"fixed-case\">DEG<\/jats:styled-content>s), followed by determination of their expression in noncancerous tissues and <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content> specimens. Next, weighted gene co\u2010expression network analysis (<jats:styled-content style=\"fixed-case\">WGCNA<\/jats:styled-content>) was conducted on <jats:styled-content style=\"fixed-case\">DEG<\/jats:styled-content>s to obtain tumor\u2010associated gene modules. Genes in those modules were intersected with <jats:styled-content style=\"fixed-case\">DEG<\/jats:styled-content>s for gene ontology and Kyoto Encyclopedia of Genes and Genomes functional enrichment analysis. Then protein\u2010protein interaction network of tumor\u2010associated genes was constructed to select genes most closely linked to <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content>. Afterward, expression of chromosome 9 open reading frame 24 (c9orf24), primary ciliary dyskinesia protein 1 (<jats:styled-content style=\"fixed-case\">PCDP<\/jats:styled-content>1), and leucine\u2010rich repeat\u2010containing protein 46 (<jats:styled-content style=\"fixed-case\">LRRC<\/jats:styled-content>46) was detected in <jats:styled-content style=\"fixed-case\">GSE<\/jats:styled-content>53819 and further verified in <jats:styled-content style=\"fixed-case\">GSE<\/jats:styled-content>12452 and <jats:styled-content style=\"fixed-case\">GSE<\/jats:styled-content>64634. Differential analysis on <jats:styled-content style=\"fixed-case\">GSE<\/jats:styled-content>53819 found that 2,173 genes were aberrantly expressed in <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content>, among which 917 genes are upregulated and 1,256 genes are downregulated. <jats:styled-content style=\"fixed-case\">WGCNA<\/jats:styled-content> showed that genes were enriched in 17 modules and 727 genes exhibited ectopic expression in <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content> and enriched in cytokine\u2010cytokine receptor interaction,\u00a0cytochrome P450, and chemical carcinogenesis signaling pathways, among which c9orf24, <jats:styled-content style=\"fixed-case\">PCDP<\/jats:styled-content>1, and <jats:styled-content style=\"fixed-case\">LRRC<\/jats:styled-content>46 were poorly expressed in <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content>. Therefore, c9orf24, <jats:styled-content style=\"fixed-case\">PCDP<\/jats:styled-content>1, and <jats:styled-content style=\"fixed-case\">LRRC<\/jats:styled-content>46 might serve as prominent diagnostic markers for <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content>, which presents new insights for <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content> therapy.<\/jats:p>","DOI":"10.1111\/cts.12690","type":"journal-article","created":{"date-parts":[[2019,12,21]],"date-time":"2019-12-21T10:43:35Z","timestamp":1576925015000},"page":"265-274","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Identification of Potential Therapeutic Gene Markers in Nasopharyngeal Carcinoma Based on Bioinformatics Analysis"],"prefix":"10.1111","volume":"13","author":[{"given":"Kai","family":"Xue","sequence":"first","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery The Second Hospital Jilin University Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinfeng","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology The Second Hospital Jilin University Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Gynaecology and Obstetrics The Second Hospital Jilin University Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery The Second Hospital Jilin University Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery The Second Hospital Jilin University Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunshun","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery The Second Hospital Jilin University Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengbi","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery The Second Hospital Jilin University Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,12,21]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.947"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.20833"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2014.02.006"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40880-017-0257-9"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.soc.2015.03.008"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/13543784.2017.1324568"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-017-2496-1"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.7150\/jca.17317"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdq277"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.4193\/Rhin11.239"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.7150\/jca.15258"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2011.272"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2011.05.016"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.4149\/neo_2014_023"},{"key":"e_1_2_10_15_1","first-page":"534","article-title":"[Expression and significance of MTA1 and RECK gene in nasopharyngeal carcinoma]","volume":"25","author":"Deng Y.","year":"2011","journal-title":"Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10985-007-9065-x"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v046.i11"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2011.0118"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anl.2011.02.012"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2015.60.9347"},{"key":"e_1_2_10_21_1","first-page":"37","article-title":"Emerging treatment options for nasopharyngeal carcinoma","volume":"7","author":"Zhang L.","year":"2013","journal-title":"Drug Des. 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