{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T17:26:25Z","timestamp":1789233985550,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,4,7]],"date-time":"2020-04-07T00:00:00Z","timestamp":1586217600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["ascpt.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Translational Sci"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:p>Detecting patients with dihydropyrimidine dehydrogenase (DPD) deficiency is becoming a major concern in clinical oncology. Monitoring physiologic plasma uracil and\/or plasma uracil\u2010to\u2010dihydrouracil metabolic ratio is a common surrogate frequently used to determine DPD phenotype without direct measurement of the enzymatic activity. With respect to the increasing number of patients rquiring analysis, it is critical to develop simple, rapid, and affordable methods suitable for routine screening. We have developed and validated a simple and robust ultraperformance liquid chromatography\u2012ultraviolet (UPLC\u2010UV) method with shortened (i.e., 12\u00a0minutes) analytical run\u2010times, compatible with the requirements of large\u2010scale upfront screening. The method enables detection of uracil (U) over a range of 5\u2013500\u00a0ng\/ml (265\u00a0nm) and of dihydrouracil (UH2) over a range of 40\u2013500\u00a0ng\/ml (210\u00a0nm) in plasma with no chromatographic interference. When used as part of routine screening for DPD deficiency, this method was fully able to discriminate nondeficient patients (i.e., with U levels &lt; 16\u00a0ng\/ml) from deficient patients at risk of severe toxicity (i.e., U\u00a0&gt;\u00a016\u00a0ng\/ml). Results from 1 month of routine testing are presented and, although no complete deficits were detected, 10.7% of the screened patients presented DPD deficiency and would thus require s decresed dose. Overall, this new method, using a simple preanalytical solid\u2010phase extraction procedure, and based on use of a standard UPLC apparatus, is both cost\u2010 and time\u2010effective and can be easily implemented in any laboratory aiming to begin routine DPD testing.<\/jats:p>","DOI":"10.1111\/cts.12762","type":"journal-article","created":{"date-parts":[[2020,2,14]],"date-time":"2020-02-14T15:40:40Z","timestamp":1581694840000},"page":"761-768","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["A Simple and Rapid UPLC\u2010UV Method for Detecting DPD Deficiency in Patients With Cancer"],"prefix":"10.1111","volume":"13","author":[{"given":"Cl\u00e9mence","family":"Marin","sequence":"first","affiliation":[{"name":"Laboratoire de Pharmacologie et Toxicologie La Timone University Hospital of Marseille Assistance Publique H\u00f4pitaux de Marseille Marseille France"},{"name":"SMARTc Unit Center de Recherche en Canc\u00e9rologie de Marseille UMR Inserm U1068 Aix Marseille Universitaire Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anis","family":"Krache","sequence":"additional","affiliation":[{"name":"SMARTc Unit Center de Recherche en Canc\u00e9rologie de Marseille UMR Inserm U1068 Aix Marseille Universitaire Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chlo\u00e9","family":"Palmaro","sequence":"additional","affiliation":[{"name":"Laboratoire de Pharmacologie et Toxicologie La Timone University Hospital of Marseille Assistance Publique H\u00f4pitaux de Marseille Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathilde","family":"Lucas","sequence":"additional","affiliation":[{"name":"Laboratoire de Pharmacologie et Toxicologie La Timone University Hospital of Marseille Assistance Publique H\u00f4pitaux de Marseille Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentin","family":"Hilaire","sequence":"additional","affiliation":[{"name":"Laboratoire de Pharmacologie et Toxicologie La Timone University Hospital of Marseille Assistance Publique H\u00f4pitaux de Marseille Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e9e","family":"Ugdonne","sequence":"additional","affiliation":[{"name":"Laboratoire de Pharmacologie et Toxicologie La Timone University Hospital of Marseille Assistance Publique H\u00f4pitaux de Marseille Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e9n\u00e9dicte","family":"De Victor","sequence":"additional","affiliation":[{"name":"Laboratoire de Pharmacologie et Toxicologie La Timone University Hospital of Marseille Assistance Publique H\u00f4pitaux de Marseille Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Quaranta","sequence":"additional","affiliation":[{"name":"Laboratoire de Pharmacologie et Toxicologie La Timone University Hospital of Marseille Assistance Publique H\u00f4pitaux de Marseille Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Solas","sequence":"additional","affiliation":[{"name":"Laboratoire de Pharmacologie et Toxicologie La Timone University Hospital of Marseille Assistance Publique H\u00f4pitaux de Marseille Marseille France"},{"name":"SMARTc Unit Center de Recherche en Canc\u00e9rologie de Marseille UMR Inserm U1068 Aix Marseille Universitaire Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Lacarelle","sequence":"additional","affiliation":[{"name":"Laboratoire de Pharmacologie et Toxicologie La Timone University Hospital of Marseille Assistance Publique H\u00f4pitaux de Marseille Marseille France"},{"name":"SMARTc Unit Center de Recherche en Canc\u00e9rologie de Marseille UMR Inserm U1068 Aix Marseille Universitaire Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Ciccolini","sequence":"additional","affiliation":[{"name":"Laboratoire de Pharmacologie et Toxicologie La Timone University Hospital of Marseille Assistance Publique H\u00f4pitaux de Marseille Marseille France"},{"name":"SMARTc Unit Center de Recherche en Canc\u00e9rologie de Marseille UMR Inserm U1068 Aix Marseille Universitaire Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,4,7]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.7991"},{"key":"e_1_2_9_2_1","first-page":"2028","article-title":"Pharmacogenetics and metabolism from science to implementation in clinical practice: the example of dihydropyrimidine dehydrogenase","volume":"23","author":"Del Re M.","year":"2017","journal-title":"Curr. 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