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We labeled myelin in vivo with deuterium, and modeled kinetics of myelin breakdown products \u03b2\u2010galactosylceramide (\u03b2\u2010GalC) and N\u2010Octadecanoyl\u2010sulfatide (NO\u2010Sulf). Five patients with MS received 120\u00a0ml 70% D<jats:sub>2<\/jats:sub>O daily for 70\u00a0days and were compared with six healthy subjects who previously received the same procedure. Mass spectrometry and compartmental modeling were used to quantify the turnover rate of \u03b2\u2010GalC and NO\u2010Sulf in cerebrospinal fluid (CSF). Turnover rate constants of the fractions of \u03b2\u2010GalC and NO\u2010Sulf with non\u2010negligible turnover were 0.00186 and 0.00714, respectively, in both healthy subjects and patients with MS. The turnover half\u2010life of \u03b2\u2010GalC and NO\u2010Sulf was calculated as 373\u00a0days and 96.5\u00a0days, respectively. The effect of MS on the NO\u2010Sulf (49.4% lower fraction with non\u2010negligible turnover) was more pronounced compared to the effect on \u03b2\u2010GalC turnover (18.3% lower fraction with non\u2010negligible turnover). Kinetics of myelin breakdown products in the CSF are different in patients with MS compared with healthy subjects. This may be caused by slower myelin production in these patients, by a higher level of degradation of a more stable component of myelin, or, most likely, by a combination of these two processes. Labeling myelin breakdown products is a useful method that can be used to quantify myelin turnover in patients with progressive MS and can therefore be used in proof\u2010of\u2010concept studies with remyelination therapies.<\/jats:p>","DOI":"10.1111\/cts.13181","type":"journal-article","created":{"date-parts":[[2021,11,20]],"date-time":"2021-11-20T06:52:45Z","timestamp":1637391165000},"page":"638-648","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Kinetics of myelin breakdown products: A labeling study in patients with progressive multiple sclerosis"],"prefix":"10.1111","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4503-6467","authenticated-orcid":false,"given":"Kawita M. 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