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The objective of the study was to compare Chinese patients with 5\u03b1\u2010RD2, AIS, and healthy men. Sixteen patients with 5\u03b1\u2010RD2, 10 patients with AIS, and 39 healthy men were included. Serum androgen profiles were compared in these subjects using liquid chromatography\/tandem mass spectrometry (LC\u2010MS\/MS). Based on clinical features and laboratory tests, 5\u03b1\u2010RD2 and AIS were diagnosed and confirmed by genotyping. Dihydrotestosterone (DHT) and testosterone (T) were both significantly lower in patients with 5\u03b1\u2010RD2 than AIS (<jats:italic>p<\/jats:italic> &lt; 0.0001). The T\/DHT ratio was higher in 5\u03b1\u2010RD2 (4.5\u201388.6) than AIS (13.4\u201326.7) or healthy men (7.6\u201340.5). Using LC\u2010MS\/MS, a cutoff T\/DHT value of 27.3 correctly diagnosed 5\u03b1\u2010RD2 versus AIS with sensitivity 93.8% and specificity 100%. Among the adrenal\u2010derived 11\u2010oxygenated androgens, 11\u03b2\u2010hydroxyandrostenedione (11OHA4) and 11\u2010ketoandrostenedione (11KA4) were also lower in patients with 5\u03b1\u2010RD2 than those of patients with AIS. In contrast, 11\u03b2\u2010hydroxytestosterone (11OHT) was higher in 5\u03b1\u2010RD2 than AIS. Furthermore, a 11OHT\/11OHA4 cutoff value of 0.048 could also distinguish 5\u03b1\u2010RD2 from AIS. Thus, both elevated T\/DHT values above 27.3 and the unexpected 11\u2010oxygenated androgen profile, with a 11OHT\/11OHA4 ratio greater than 0.048, distinguished 5\u03b1\u2010RD2 from AIS. These data suggest that the metabolism of both gonadal and adrenal\u2010derived androgens is altered in 5\u03b1\u2010RD2.<\/jats:p>","DOI":"10.1111\/cts.13184","type":"journal-article","created":{"date-parts":[[2021,11,10]],"date-time":"2021-11-10T08:31:18Z","timestamp":1636533078000},"page":"658-666","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Differences of adrenal\u2010derived androgens in 5\u03b1\u2010reductase deficiency versus androgen insensitivity syndrome"],"prefix":"10.1111","volume":"15","author":[{"given":"Bing","family":"Han","sequence":"first","affiliation":[{"name":"Department of Endocrinology Shanghai Ninth People\u2019s Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Endocrinology Shanghai Ninth People\u2019s Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haijun","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Urology Shanghai Ninth People\u2019s Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianwei","family":"Ren","sequence":"additional","affiliation":[{"name":"Division of Metabolism, Endocrinology and Diabetes Department of Internal Medicine Department of Pharmacology the Program for Disorders of Sexual Development University of Michigan  Ann Arbor Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"O\u2019Day","sequence":"additional","affiliation":[{"name":"Division of Metabolism, Endocrinology and Diabetes Department of Internal Medicine Department of Pharmacology the Program for Disorders of Sexual Development University of Michigan  Ann Arbor Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Endocrinology Shanghai Ninth People\u2019s Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjiao","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Endocrinology Shanghai Ninth People\u2019s Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tong","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Endocrinology Shanghai Ninth People\u2019s Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard J.","family":"Auchus","sequence":"additional","affiliation":[{"name":"Division of Metabolism, Endocrinology and Diabetes Department of Internal Medicine Department of Pharmacology the Program for Disorders of Sexual Development University of Michigan  Ann Arbor Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Qiao","sequence":"additional","affiliation":[{"name":"Department of Endocrinology Shanghai Ninth People\u2019s Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,11,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2016.05.020"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/humu.22046"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2016-1990"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2014-2605"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00431-010-1189-4"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/cen.12456"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/cen.12799"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(02)00368-4"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2265.2003.01763.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2010-1024"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000313372"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40618-018-0940-y"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1530\/EJE-15-1181"},{"key":"e_1_2_9_15_1","volume-title":"Correlations of radiographic and hormonal indices of disease control in a cohort of children and adults with classic 21\u2010hydroxylase deficiency. 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