{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T01:44:40Z","timestamp":1777081480844,"version":"3.51.4"},"reference-count":52,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T00:00:00Z","timestamp":1696204800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["ascpt.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Translational Sci"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Aberrant autophagic activity is observed in osteoarthritic joints. Vitamin D was shown to alleviate not only osteoarthritis severity, but also autophagy process. However, the influence of vitamin D on autophagy in knee osteoarthritis (KOA) remains ambiguous. This study aimed to determine the effect of vitamin D<jats:sub>2<\/jats:sub> on serum levels of autophagosome protein LC3A in patients with KOA and whether LC3A levels were correlated with serum 25\u2010hydroxyvitamin D (25(OH)D) and clinical outcomes of patients with KOA. A total of 165 patients with KOA and 25 healthy controls were recruited. Vitamin D<jats:sub>2<\/jats:sub> (ergocalciferol) was administered to patients with KOA at a weekly dosage of 40,000\u2009IU. Serum LC3A, knee pain and functional scores, muscle strength, physical performance, and biochemical parameters were examined before and after 6\u2009months of vitamin D<jats:sub>2<\/jats:sub> supplementation. Serum LC3A levels were significantly higher in patients with KOA than healthy controls. In patients with KOA, vitamin D<jats:sub>2<\/jats:sub> supplementation significantly decreased serum LC3A levels. Furthermore, baseline levels of serum LC3A were significantly associated with radiographic severity, pain and functional scores, total cholesterol, hs\u2010CRP, IL\u20106, protein carbonyl, and serum 25(OH)D. After adjusting for established confounders, independent relationships among serum LC3A and radiographic severity, pain and functional scores, total cholesterol, hs\u2010CRP, IL\u20106, protein carbonyl, and serum 25(OH)D were also observed. Vitamin D<jats:sub>2<\/jats:sub> supplementation was shown to not only decrease serum levels of LC3A, inflammatory markers, as well as oxidative stress, but also improve muscle strength and physical performance in patients with KOA.<\/jats:p>","DOI":"10.1111\/cts.13646","type":"journal-article","created":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T03:38:35Z","timestamp":1695699515000},"page":"2543-2556","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Effect of vitamin D supplementation on circulating level of autophagosome protein <scp>LC3A<\/scp>, inflammation, and physical performance in knee osteoarthritis"],"prefix":"10.1111","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-8748-7869","authenticated-orcid":false,"given":"Wacharapol","family":"Saengsiwaritt","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy Mahidol University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4987-3497","authenticated-orcid":false,"given":"Jiraphun","family":"Jittikoon","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy Mahidol University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9457-9823","authenticated-orcid":false,"given":"Usa","family":"Chaikledkaew","sequence":"additional","affiliation":[{"name":"Social and Administrative Pharmacy Division, Department of Pharmacy, Faculty of Pharmacy Mahidol University  Bangkok Thailand"},{"name":"Mahidol University Health Technology Assessment (MUHTA) Graduate Program Mahidol University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9366-8039","authenticated-orcid":false,"given":"Tulyapruek","family":"Tawonsawatruk","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Faculty of Medicine, Ramathibodi Hospital Mahidol University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3852-9092","authenticated-orcid":false,"given":"Sittisak","family":"Honsawek","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Center of Excellence in Osteoarthritis and Musculoskeleton, Faculty of Medicine and King Chulalongkorn Memorial Hospital, Thai Red Cross Society Chulalongkorn University  Bangkok Thailand"},{"name":"Department of Orthopaedics, Vinai Parkpian Orthopaedic Research Center, Faculty of Medicine, King Chulalongkorn Memorial Hospital, Thai Red Cross Society Chulalongkorn University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1132-7442","authenticated-orcid":false,"given":"Wanvisa","family":"Udomsinprasert","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy Mahidol University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,10,2]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2016.72"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/acr.24131"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/1947603521990866"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00005-020-00580-8"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes11080854"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra2022774"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.09.048"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/1941738109350438"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/5171602"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom12101357"},{"issue":"18","key":"e_1_2_13_12_1","first-page":"9246","article-title":"Sirt7 protects chondrocytes degeneration in osteoarthritis via autophagy activation","volume":"24","author":"Wu SY","year":"2020","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.34323"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2019.03.071"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2012.164487"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2021.108841"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu9080799"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/acr2.1042"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2023.1101516"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12889-021-11888-1"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11999-016-4732-4"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2011-0385"},{"key":"e_1_2_13_23_1","first-page":"161","volume-title":"Methods in Biological Oxidative Stress. Methods in Pharmacology and Toxicology","author":"Castegna A","year":"2003"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1213\/ANE.0000000000002864"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncprheum0534"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2016.148"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2021108863"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.4161\/auto.7.5.14226"},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2022.09.005"},{"key":"e_1_2_13_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/os.13057"},{"key":"e_1_2_13_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-013-0811-0"},{"key":"e_1_2_13_32_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2017.2934"},{"key":"e_1_2_13_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.39073"},{"key":"e_1_2_13_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.27305"},{"key":"e_1_2_13_35_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2013.1520"},{"key":"e_1_2_13_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbspin.2015.06.009"},{"key":"e_1_2_13_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-010-0458-z"},{"key":"e_1_2_13_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcot.2018.05.012"},{"key":"e_1_2_13_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.21601"},{"issue":"8","key":"e_1_2_13_40_1","first-page":"466","article-title":"Restorative effect of vitamin D deficiency on knee pain and quadriceps muscle strength in knee osteoarthritis","volume":"53","author":"Heidari B","year":"2015","journal-title":"Acta Med Iran"},{"key":"e_1_2_13_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11999-013-3201-6"},{"key":"e_1_2_13_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40520-020-01778-8"},{"key":"e_1_2_13_43_1","doi-asserted-by":"publisher","DOI":"10.4172\/2167-7921.1000222"},{"key":"e_1_2_13_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2016.05.020"},{"key":"e_1_2_13_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2019.02.114"},{"key":"e_1_2_13_46_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/6403504"},{"key":"e_1_2_13_47_1","doi-asserted-by":"publisher","DOI":"10.1139\/bcb-2019-0333"},{"key":"e_1_2_13_48_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2011-200557"},{"key":"e_1_2_13_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.01.130"},{"key":"e_1_2_13_50_1","doi-asserted-by":"publisher","DOI":"10.4110\/in.2022.22.e34"},{"key":"e_1_2_13_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbspin.2007.02.002"},{"key":"e_1_2_13_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00296-006-0247-8"},{"key":"e_1_2_13_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.21387"}],"updated-by":[{"DOI":"10.1111\/cts.70324","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T00:00:00Z","timestamp":1754870400000}},{"DOI":"10.1111\/cts.13856","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T00:00:00Z","timestamp":1716940800000}}],"container-title":["Clinical and Translational Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/ascpt.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cts.13646","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,21]],"date-time":"2024-01-21T12:33:43Z","timestamp":1705840423000},"score":1,"resource":{"primary":{"URL":"https:\/\/ascpt.onlinelibrary.wiley.com\/doi\/10.1111\/cts.13646"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,2]]},"references-count":52,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2023,12]]}},"alternative-id":["10.1111\/cts.13646"],"URL":"https:\/\/doi.org\/10.1111\/cts.13646","archive":["Portico"],"relation":{"correction":[{"id-type":"doi","id":"10.1111\/cts.13856","asserted-by":"object"}]},"ISSN":["1752-8054","1752-8062"],"issn-type":[{"value":"1752-8054","type":"print"},{"value":"1752-8062","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,2]]},"assertion":[{"value":"2023-06-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}